Belo, Isabel

Braga, Adelaide

Crutz-Le Coq, A. M.

Dulermo, R.

Nicaud, J. M.

Universidade do Minho: RepositoriUM

Comparison of  y-decalactone production from castor oil by Y. lipolytica mutants in batch and step-wise fed-batch cultures

O biocat2014 ~ HAMBURG Poster Enzymatic and whole cell biotransformations P3-32 Comparison of y-decalactone production from castor oil by Y. lipolytica mutants in batch and step-wise fed-batch cultures A. Braga 1, A. M. Crutz-Le Coq2, R. Dulermo2, J. - M. Nicaud2, I. Belo 1 "centre of Biological Engineering, University of Minho, Braga, Portugal; 21NRA, UMR1319, MICALIS, AgroParisTech, Centre de Biotechnologie Agro-lndustrielle , Thiverval-Grignon, France Developments of y-decalactone production processes by ricinoleic acid biotransformation ave been made with the wild-type strain, namely Yarrowia /ipolytica W29. Nevertheless, a apidly lactone degradation is observed due to the high level of acyl-CoA oxidase activity in Y. lipolytica [1, 2]. The purpose of this work is to monitor the performance of strains with modifications in the "pid metabolism at the ~-oxidation pathway (acyl-CoA oxidases) and the triglyceride ydrolysis (LIP2 overexpression [3]). Lactone production was followed in batch and step-wise "'ad-batch cultures using castor oil as substrate in a 4 L bioreactor. The y-decalactone oduction and degradation in the wild-type strain W29 (ATCC20460) and mutant strains LY40-2P (tipox2-5, pPOX2-POX2), JMY3010 (WT, pTEF-L/P2) will be reported . ..... epending on genotype, degradation of the y-decalactone was prevented. Also, a faster · ial rate of aroma production was obtained with strain overexpressing LIP2 due to the fast - •drolysis of castor oil and release of ricinoleic acid. Step-wise fed-batch cultures improved -decalactone production only for MTL Y40-2P strain, for which a 1.6-fold increase in -decalactone final concentration (7 g/L) was achieved. -. Wache Y, Laroche C, Bergmark K, Mollet"-Andersen C, Agueda M, Le Dall, M-T, Wang H, Nicaud JM, Belin JM. 2000. Involvement of Acyl Coenzyme A oxidase lsozymes in biotransformation of methyl ricinoleate into y-decalactone by Yarrowia lipolytica. Applied and Environmental Microbiology. 66:1233-1236. Wache Y, Agueda M, Choquet A, Gatfield IL, Nicaud JM, Belin JM. 2001. Role of ~-oxidation enzymes in y-decalactone production by the yeast Yarrowia lipolytica. Applied and Environmental Microbiology. 67:5700-5704. Pignede G., Wang, H., Fudalej F., Gaillardin C, Seman M. Nicaud J-M. 2000 Characterization of an extracellular lipase encoded by LIP2 in Yarrowia lipolytica. J. Bacterial. 182:2802-2810. - a ·anal Congress on Biocatalysis :· - September 4, 2014, Hamburg, Germany 193 

Comparison of y-decalactone production from castor oil by Y. lipolytica mutants in batch and step-wise fed-batch cultures

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Universidade do Minho: RepositoriUM

Universidade do Minho: RepositoriUM