[Excerpt] Filamentous fungi are ubiquitous organisms and can easily be isolated from a wide
range of environment. Some efforts have been made to investigate microbial
communities in marine sponges of eukaryotes using both approaches of conventional
taxonomy (morphology) and molecular taxonomy (Passarini et al. 2011). The
classification of some filamentous fungi taxa can be difficult to achieve based on these
methods. Currently, Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Intact
Cell Mass Spectrometry (MALDI-TOF ICMS) has been widely used to identify and
characterise a large number of taxa of filamentous fungi (Santos et al. 2010). The aim of
this study was to investigate the diversity of 33 Trichoderma isolated from the southern
Atlantic Ocean marine sponge Dragmacidon reticulata using a polyphasic approach
