Baermann

Blatt

C. VILA-VERDE

D. S. NUNES

H. T. GONZAGA

Holm

J. M. COSTA-CRUZ

J. P. CUNHA-JÚNIOR

Koosha

Lowry

Mangali

Martinez

Moraes

Rossi

Rugai

V. S. RIBEIRO

SUMMARYThe aim of this study was to fractionate and partially characterize the antigenic extract of filariform larvae ofStrongyloides venezuelensisin ion-exchange resin diethylaminoethyl sepharose (DEAE), to obtain antigenic fractions potentially applicable in immunoassays. Somatic antigen (SA) and its fractions DEAE S1 and DEAE S2 - which interacted with the resin - were evaluated by 1-dimensional electrophoresis to obtain protein profiles. SA and its fractions were tested in serum samples for IgG detection by ELISA. Serum samples (n = 155) were analysed: 50 from strongyloidiasis patients (G1), 55 from patients with other parasitic infections (G2) and 50 from healthy volunteers. Sensitivity (Se), specificity (Sp), area under curve (AUC) and likelihood ratios (LR) were calculated. The DEAE S2 fraction provided a high diagnostic value for IgG detection (Se 92·0%, Sp 91·4%, AUC 0·981, LR+ 10·75, LR − 0·09). In conclusion, the DEAE S2 fraction would probably be a source of immunodominant polypeptides for IgG detection in human strongyloidiasis serodiagnosis.</jats:p

Crossref

Parasitology

Ion-exchange protocol to obtain antigenic fractions with potential for serodiagnosis of strongyloidiasis

Ion-exchange protocol to obtain antigenic fractions with potential for serodiagnosis of strongyloidiasis

Abstract

Similar works

Full text

Available Versions

Crossref