Fungal inocula are used for biotechnological applications especially in bioremediation process.
They are formulated with a carrier, nutrient sources, binder, and lubricant to be encapsulated for
a layer of fungal mycelium. The white-rot fungus Anthracophyllum discolor was studied for its
ability to growth actively to form these inocula and to produce ligninolytic enzymes. In this study,
fungal inocula were formulated on 3 different mixtures of lignocellulosic materials: F1, F2 and F3
and, two kind of fungal inocula were produced, coated and uncoated. The ligninolytic enzymes
Laccase (Lac), Manganese Peroxidase (MnP), Manganese-independent Peroxidase (MiP) and
Lignin Peroxidase (LiP) were measured during the experiments. The main enzymatic activity
detected was Manganese Peroxidase for F1, F2 and, F3 tested and Laccase had the lowest
activity. The amount of total ligninolytic enzyme activity was approximately 40% higher on
uncoated than coated pellets, demonstrating the capacity of Anthracophy/lum discolor to growth
and produce enzymes on this formulation. Uncoated pellet showed the highest MnP activity,
after 15 days in average was 477,13 mmol/min/g being the pick of enzyme at the day 15 with
220,99 mmol/min/g. In contrast for coated pellets the MnP activity was 271,15 mmol/min/g. The
UP was not detected on fungal inocula studied. Based on the results of ligninolytic enzymes, the
fungal ability to growth and colonization on these pellet formulations showed by scanning
electron microscopy (SEM), the pellet uncoated formulated with F1 lignocellulosic materials
presents high potential to be used on bioremediation processes. These results will be presented
and discussed
