Since bacteria are continuously acquiring resistance to
conventional chemical agents, it is urgently needed the
development of new strategies for biofilm control. It is
well recognised that certain microorganisms represent
an important source of novel biologically active
compounds, with pronounced antibacterial activity, as
secondary metabolites. Such substances are accepted to
be essential for their producers, inhibiting other bacteria
that compete for common resources. The main goal of this
work was to investigate the antimicrobial effect of secondary
metabolites secreted by P. aeruginosa on planktonic and
sessile growth of several pathogens, in order to later use those
molecules as bio-regulation agents. P. aeruginosa
supernatants had potential as anti-biofilm agents but
only against staphylococcal biofilms since they failed in
disturb other biofilm consortia that encompassed Grambacteria.
This trait makes them quite ineffective
chemical countermeasures against real biofilms
