Recombinant protein production is a useful technology
for therapeutic and diagnostic applications. The
bacterium Escherichia coli is widely used for the bioproduction
of proteins, but still presents some drawbacks.
Recombinant proteins with biomedical interest have
proved difficult to express properly in this host system,
resulting in insoluble protein aggregates. Fusion protein
technology has been applied to overcome these
drawbacks and to optimize protein expression in E. coli.
This research aims at the evaluation of a novel fusion
system (Fh8 and H tags) effects on protein production.
Target proteins used in this work present biomedical
interest and results obtained here showed that these novel
fusion tags increased protein production yields. This
novel fusion system is a promising tool for the
production of recombinant proteins with biomedical
interest
