Staphylococcus epidermidisis a common commensal coloniser of the human skin and is currently the most
frequent cause of biomaterial associated infections. Several studies have attempted to identify the
determinants that distinguish invasive from commensals S. epidermidisstrains. Its pathogenesis is directly
related to its ability to establish multi-layered and highly structured biofilms, resistant to antimicrobial
agents. This bacterium expresses several factors that are responsible for the development of the biofilm,
including the contribution of specific factors (icaA, aap and bhp genes) in the accumulation phase.
Recently, several research groups have been trying to understand the contribution of the genes involved
in biofilm formation. Thus, the main goal was to analyse the gene expression of icaA, aap and bhp and
compare with the formation of the biofilm structure. Two S. epidermidis strains, a clinical and a
commensal were characterized at the level of biofilm formation, at different incubation times. According
to our results both strains showed an increase of biomass production overtime, revealing the importance
to use screening assays with more than 24 h of incubation. A biofilm structure analysis was also performed
to detect the presence of poly-N-acetylglucosamine (PNAG), the major component of S. epidermidis
biofilm matrix. The results revealed a higher production of PNAG only after 48 h for SECOMO034.A1.
Finally, the gene expression at two different incubation times was determined, confirming the importance
of the icaA gene in the accumulation stage, explaining the high production of biomass and PNAG. On the
other hand, the aap and bhp expression levels raised some questions about their role in the biofilm
process