Alves, D.

Lopes, Susana Patrícia

Pereira, Maria Olívia

Universidade do Minho: RepositoriUM

A colistin coating to prevent biomaterial-associated infectionsDiana Alves, Susana Lopes and Maria Olívia Pereira*dianalves@ceb.uminho.pt*IBB-Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho,Campus de Gualtar 4710-057, Braga, Portugal IntroductionBacterial colonisation of indwelling devices followed by biofilm formation remains a serious concern in modern health care.Device-associated infections are difficult to treat because cells within a biofilm are less susceptible to antimicrobial treatmentand to host immune system. The emergence of multidrug resistant bacteria and the lack of alternative therapeutic optionshave led to the revival of colistin. Although effective, some concerns have been raised about its toxicity and the developmentof bacterial resistance. Colistin covalent immobilization onto a biomaterial surface may overcome these drawbacks as it avoidspatient exposure to sub-inhibitory concentrations.MethodsDopamineBicine pH 8.5Polydopamine film on PCColistinColistin coated PCPCDopamine + ColistinColistin coated PCPCAcknowledgments: IBB-CEB and FCT, for the financial support: PhD Grant SFRH/BD/78063/2011 and project  PTDC/SAUSAP/113196/2009 / FCOMP-01-0124-FEDER-016012One-step approachTwo-step approachResultsSURFACE CHARACTERIZATIONtwo-step approach one-step approach0.00.40.81.2PC pDA ColistinFraction of death bacteriatwo-step approach one-step approach0.00.40.81.2Fraction of death bacteriaPC pDAColistinANTIMICROBIAL PERFORMANCEConclusionsAIMto apply and optimize a polydopamine (pDA) dip-coating strategy for covalent immobilization of colistin on polycarbonate (PC) surfacesCOLISTIN is a promising candidate for the development of an ANTIMICROBIAL COATING for clinical applications. 100 µm Polydopamine was successfully exploited to functionalize biomaterial surfaces with colistin to impart them withantimicrobial properties. Colistin-coated surfaces were effective at killing bacteria on contact. P. aeruginosa ATCC 27853 P. aeruginosa ATCC 39324

A colistin coating to prevent biomaterial-associated infections

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Universidade do Minho: RepositoriUM