In this study five clinical isolates strains were used, and nine antibiotics at breakpoint concentrations:
vancomycin, tetracycline, rifampicin, gentamicin, cefazolin, cephalothin, levofloxacine, daptomycin and
clindamycin were tested. 48 hours biofilms were grown on Calgary Biofilm Device (CBD) and challenged
overnight with antibiotics alone and in combination. Biofilm cells viability was determined by colony forming
units (cfu). Afterwards, the effect of the most active antibiotics combinations against S. epidermidis biofilm on
genetic expression of some genes of interest such as: icaA, icaR, sarA and rsbU was determined by real-time PCR.
Although biofilms were generally insensitive to individual antibiotics, they were more susceptible to
combinations. Levofloxacine was a constituent of almost all the combinations active against S. epidermidis
biofilm pointing to be part of any antibiotic therapy directed against biofilms of these organisms
