Infective endocarditis is a serious clinical issue, often related to the prolonged use of intravenous catheters
that can eventually become infected. Although polymicrobial endocarditis (PE) involving Staphylococcus
epidermidis and Candida species is uncommon, it is generally associated with patients’ high mortality due to
the presence of Candida spp.. Additionally, investigation on the phenomenon of microbial competition in PE
is still scarce. Thus, the main goal of this work is to deepen the knowledge of PE related to Staphylococcus and
Candida spp., by studying the competition and the influence of each species on biofilm formation on silicone.
Both strains of S. epidermidis and C. parapsilosis were clinical isolates. Biofilm assays were performed on silicone
coupons in 24-well plates, for 24, 48, 72h and 8 days. Total biomass was measured by crystal violet staining
while cell viability was evaluated through colony forming units (CFU) enumeration. Species differentiation
in mixed biofilms was achieved using selective mediums and also by Scanning Electronic Microscopy (SEM)
observation. According to the results, and comparing to single spp. biofilms, it was noticed that mixed biofilms
are not cumulative. Despite this, in all biofilm conditions an increase in the number of cells was observed after
72h. Moreover, an inhibitory effect of S. epidermidis on C. parapsilosis biofilms was noticed, especially at 72h.
In conclusion, this study provided an important approach for a better understanding of S. epidermidis and C.
parapsilosis biofilms composition, structure and interactions, which can give new insights on PE caused by
these microorganisms
