The cosmopolitan Articulospora tetracladia is a dominant sporulating species on
decomposing plant-litter in streams of Northwest Iberian Peninsula. In an attempt to
elucidate the intraspecific genetic diversity of A. tetracladia of Iberian Peninsula,
isolates were attained from various types of substrates from streams of North and
Central Portugal and North Spain, between 2000 and 2010. Genetic diversity of
these fungal populations was assessed by denaturing gradient gel electrophoresis
(DGGE) fingerprints and by using ITS1-5.8S-ITS2 barcodes. The ITS1-5.8S-ITS2
barcodes published at the National Center for Biotechnology (NCBI) or National
Institute of Technology and Evaluation Biological Resource Center (NBRC) were
retrieved to probe into the genetic diversity of A. tetracladia isolated from Iberian
Peninsula and other parts of the world (Central Europe, UK, Canada, Japan and
Malaysia). The PCR-DGGE of ITS2 region of 50 Iberian fungal isolates
distinguished 8 OTUs. The ITS sequences of 68 fungal isolates yielded nine OTUs,
but 5 fungal isolates were not assigned to any of these OTUs. The A. tetracladia
strains did not exhibit cohesiveness based on sampling date or substrate or
geographic location. Overall results indicate that, apart Malaysian genotypes, A.
tetracladia genotypes are geographically widespread irrespective of sampling time,
sites or substrates. Moreover, PCR-DGGE appeared to be a rapid tool for assessing
intraspecific diversity of aquatic hyphomycetes.FEDER-POFC-COMPETE and FCT supported this study
(PEst-C/BIA/UI4050/2011 and PTDC/AAC-AMB/113746/2009) and SD
(SFRH/BPD/47574/2008)
