1165-1174The extract of Geitlerinema carotinosum CNP 4003 showed promising antimicrobial activity (100 and 1000 µg/disc/well) against E. coli ATCC 35218, S. aureus ATCC 25923, and M. smegmatis with inhibition zones of 6, 8, and 11 mm, respectively. In addition, the extract also exhibited cytotoxic activity (IC50 = 175 μg/ml) against mammalian (HepG2) liver cancer cell lines. Therefore, the crude extract was fractionated using column chromatography technique and the active fractions were identified. The active fractions tested against HepG2 cell line showed 95 % hemolytic activity at the concentration of 375 µg/ml with IC50 as 63 μg/ml. Further, the compounds in the active fractions were analyzed and the results indicated the presence of indoles, terpenes, and peptides. The chemical composition of the active fraction was analyzed by using gas chromatography-mass spectrometry (GC-MS). The potential antimycobacterial strain G. carotinosum CNP 4003 was confirmed by molecular characterization and the DNA sequence was deposited in the gene bank