Amaral, A. L.

Correia, M. T. S.

Ferreira, E. C.

Xavier, A. L.

Ferreira, Eugénio C.

Universidade do Minho: RepositoriUM

A.26 157 Effect of different cultivation conditions on Trametes versicolor: morphological image analysis and metabolic production  Ana M.R.B. Xavier, COPNA, Departamento de Química, Universidade de Aveiro, Campus Universitario de Santiago, 3810-193 Aveiro, Portugal; presenting author, Mariana Correia COPNA, Departamento de Química, Universidade de Aveiro, Campus Universitario de Santiago, 3810-193 Aveiro, Portugal; António Luís Amaral Instituto Superior de Engenharia de Coimbra, Instituto Politécnico de Coimbra, Rua Pedro Nunes, Quinta da Nora, 3030-199 Coimbra, Portugal; Eugénio C. Ferreira, IBB – Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710-057 Braga, Portugal   The metabolic behaviour of white rot fungus Trametes versicolor was studied on agitated submerged cultures in its ability to produce exopolysaccharides and ligninolytic enzymes. With that purpose, batch tests with four different experimental conditions were performed. Two different culture media were used namely Yeast Malt Extract Medium (Kim et al., 2002) and Trametes Defined Medium (Roy and Archibald, 1993) and the latter one also with industrial effluent addition or with xylidine addition. Laccase activity, exopolysaccharide formation and final biomass contents were determined during the batch assays and the pellets morphology was assessed by image analysis techniques. The obtained data allowed establishing the change of the metabolic pathways according to the experimental conditions, either for ligninolytic enzymatic production in the Trametes Defined Medium experiments, or for exopolysaccharides production in the Yeast Medium study. Furthermore, the image processing and analysis methodology allowed for a better comprehension of the physiological phenomena with respect to the corresponding pellets morphological stages.  Kim, S.W., Hwang, H.J., Park, J.P, Cho, Y.J., Song, C.H., Yun, J.W. (2002) Mycelial growth and exo-biopolymer production by submerged culture of various edible mushrooms under different media. Lett. Appl. Microbiol. 34, 56-61 Roy, B., Archibald, F. (1993) Effects of kraft Pulp and Lignin on Trametes versicolor Carbon Metabolism. Appl. Environ. Microbiol. 59(6) 1855-1863. 

Effect of different cultivation conditions on Trametes versicolor : morphological image analysis and metabolic production

http://repositorium.sdum.uminho.pt/bitstream/1822/9087/1/Esbes2008%5b2%5d.pdf

Effect of different cultivation conditions on Trametes versicolor : morphological image analysis and metabolic production

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Universidade do Minho: RepositoriUM