Dark fermentation hydrogen production from a mixture of food components using two different methods of methanogenic
inhibition (autoclaving and BES) and three different temperatures (37, 60, and 70 ºC) was examined in batch assays for two
different mixed anaerobic cultures - one suspended sludge (S) obtained from an anaerobic digester and one granular sludge (G)
obtained from a brewery wastewater treatment plant. In general, BES-inhibition of sludge was more robust when compared
against heat-treated inoculum. Also, hydrogen, VFA, and sCOD production were affected by increases in temperature although
the effects were less severe for G than for S. In addition, differences in individual VFAs were observed between the two inocula.
S produced more acetate as a percentage of VFATOTAL compared to G. Conversely, G produced more butyrate compared to S.
Differences in the microbial communities were likely responsible for the diverse behaviour of the two inocula.Fundação para a Ciência e a Tecnologia (FCT
