Azeredo, Joana

Gonçalves, Vânia

Henriques, Mariana

Negri, M.

Oliveira, Rosário

Silva, Sónia Carina

Williams, David

Universidade do Minho: RepositoriUM

Adhesion and biofilm formation ability by clinical urinary isolates of non-candida Albicans candida species on silicone

 58 Proceedings of Biofilms III Conference [P119] ADHESION AND BIOFILM FORMATION ABILITY BY CLINICAL URINARY ISOLATES OF NON-CANDIDA ALBICANS CANDIDA SPECIES ON SILICONE Sónia Silva*, Vânia Goncalves*, Melyssa Negri*,  Mariana Henriques*, Rosário Oliveira*, David Williams**, Joana  Azeredo* *IBB-Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar 4710-057, Braga, Portugal; **School of Dentistry, Cardiff University, Heath Park, Cardiff, CF14 4XY, United Kingdom Urinary tract infections due to Candida spp have markedly increased in the last two decades. Candida spp are now responsible for approximately 10% of urinary tract infections in hospitalized patients. In contrast to vaginal candidiasis, approximately 50% of urinary isolates of Candida are of Non-Candida albicans Candida (NCAC) spp, the most common of which are C.glabrata, C. tropicalis and C. parapsilosis spp. This has been attributed to the increased use of indwelling medical devices, such as silicone urinary catheters. Adherence of NCAC spp to surfaces is vital for biofilm formation. Consequently, formation of NCAC spp biofilms has important clinical repercussions, specifically because of their inherent tolerance to antifungal therapy and the ability of cells within biofilms to withstand host immune defences. Thus, the aim of this study was to study the adhesion and biofilm formation ability by different urinary isolates of NCAC spp on silicone.  A total of 6 strains were isolated from urinary tract, including C. parapsilosis (n=2), C. glabrata (n=2) and C. tropicalis (n=2). Reference strains of each species (C. glabrata ATCC 2001, C. tropicalis ATCC 750 and C. parapsilosis ATCC 22019) were also assayed. Adhesion and biofilm formation assays were performed incubating silicone with a cell suspension of 1.0 x107 cells/mL at 37o C. Adhesion assays were evaluated after 2 hours incubation and biofilm formation after 4, 8, 12, 24, 48 and 72 hours using the crystal violet staining method. The hydrophobicity of silicone and cell lawns was determined by contact angle measurements through the sessile drop technique. The results showed that all strains of NCAC spp examined herein presented hydrophilic cell surfaces but this property is not ruling cell adhesion to silicone. Moreover, all urinary isolates were able to adhere and form biofilm on silicone, although there were differences among species and strains. Generally, C. glabrata strains are less capable to form biofilm comparatively to C. parapsilosis and C. tropicalis strains.   The main conclusion of the present work is that NCAC spp are biofilm producers on silicone, and that the adhesion and biofilm formation ability appear to be species and strain dependent.    

http://repositorium.sdum.uminho.pt/bitstream/1822/9124/1/58%5b1%5d.pdf

Adhesion and biofilm formation ability by clinical urinary isolates of non-candida Albicans candida species on silicone

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Universidade do Minho: RepositoriUM

Universidade do Minho: RepositoriUM