Azeredo, Joana

Fernandes, Elisabete Ramos

Ferreira, E. C.

Krylov, V. N.

Nicolau, Ana

Pleteneva, E. A.

Santos, Sílvio Roberto Branco

Shaburova, O. V.

Ferreira, Eugénio C.

Universidade do Minho: RepositoriUM

74        Salmonella is an important zoonotic pathogen and remains the primary cause of reported food poisoning worldwide with massive outbreaks, being S. enteritidis the most commonly reported serovar. The increased resistance of Salmonella to antibiotics and other biocides has encouraged the development of alternatives to chemotherapy. Bacteriophages have been proved to have the ability to provide a natural, nontoxic, feasible and non-expensive control of Salmonella. In the scope of the European project Phagevet-P a Salmonella phage belonging to the Myoviridae family was isolated from a Germany (Regensburg) wastewater plant. This phage named PVP-SE-1 (previously named phage 2/2) is characterised by having a broad lytic spectrum being able to infect different Salmonella serotypes of different countries and different origins (food, environmental and clinical).   A major concern in the use of bacteriophages as biocontrol agents is their safety. As bacteriophages are amplified in their pathogenic hosts, the resultant phage product might be toxic unless efficient and adequate downstream techniques are applied. This requires a strict product control and usually high production costs. The use of a non-pathogenic host would eliminate the risk of introducing a pathogen (and possible endotoxins) in the product, increasing its safety and reducing costs once stringent effectiveness of separation and filtration processes is not required. The work summarised herein explores this possibility by characterising and comparing the lytic performance of PVP-SE-1 amplified in its respective host (a pathogenic Salmonella strain) and in a non pathogenic E. coli strain (E. coli BL21). The lytic spectra of this phage replicated in BL21 and in its original Salmonella host showed to be the same. Additionally, latency period and burst size were determined allowing for the comparison of the phages replicated in the two different hosts. The results support the possibility of employing E. coli BL21 to produce PVP-SE-1 to be used as a biocontrol agent against Salmonella.          1. Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4700-057 Braga, Portugal. 2. Institute of Genetics and Selection of Industrial Microorganisms, Moscow, 117454 Russia. Production of a Salmonella bacteriophage in a non-pathogenic E. coli strain. Sílvio R.B. Santos1*, E. Fernandes1, V. N. Krylov2, E. A. Pleteneva2, O. V. Shaburova2, A. Nicolau1, E. C. Ferreira1 and J. Azeredo1. *silviosantos@deb.uminho.pt Notes: Session IV (Phage Therapy) – Poster 2 

Production of a salmonella bacteriophage in a non-pathogenic E. coli strain

http://repositorium.sdum.uminho.pt/bitstream/1822/8279/1/EPC_74%5b1%5d.pdf

Production of a salmonella bacteriophage in a non-pathogenic E. coli strain

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Universidade do Minho: RepositoriUM

Universidade do Minho: RepositoriUM