Azeredo, Joana

Lira, Madalena

Oliveira, M. Elisabete

Oliveira, Rosário

Rodrigues, Diana Alexandra Ferreira

Santos, Lívia

Yebra-Pimentel Vilar, Eva

Universidade do Minho: RepositoriUM

EFFICACY OF WORN SILICONE HYDROGEL CONTACT LENSES DISINFECTION BY A MULTIPURPOSE SOLUTION 1Lívia Santos, 1Diana Rodrigues, 2Madalena Lira, 1Rosário Oliveira, 3Eva Yebra Pimentel-Vilar, 2M. Elisabete C. D. Real Oliveira and 1Joana Azeredo  1Centro de Engenharia Biológica, Universidade do Minho, Braga – Portugal 2Departamento de Física (Optometria), Universidade do Minho, Braga – Portugal 3Departamento de Óptica e Optometria, Universidade de Santiago de Compostela, Santiago de Compostela, Espanha  The ocular environment is challenged by several microbes from the conjunctiva or skin as from the environment. When a CL is placed in the eye microbes tend to adhere to its surface making the cornea more vulnerable to infections. To maintain the normal eye ecology, CL disinfection is an essential procedure.  The aim of this work is to evaluate the disinfection capability of a multipurpose solution (MS) to silicone hydrogel CL previously worn by human subjects.  Tested CL were Balafilcon A, Galyfilcon A, Lotrafilcon A and Lotrafilcon B. The MS was Renu Multiplus® (Baush&Lomb). Previous to the disinfection experiments CL were colonised with the strain Staphylococcus epidermidis 9142. The MS disinfection capability was evaluated through the degree of removed bacteria using a parallel plate flow chamber. The remaining cells were stained with propidium-iodide to verify their viability.  The MS was capable to remove the adhered cells except for Balafilcon A (p=0.144) and Lotrafilcon B (p=0.522) which were found through contact angle measurements and AFM to be very hydrophobic and rougher. Nevertheless, staining with propidium-iodide revealed that a great amount of the remaining adhered cells were not viable (Balafilcon A (61%) and Lotrafilcon B (53%)).  The MS seems efficient in removing bacteria from less hydrophobic and smoother surfaces. In our concern MS provides an appropriated maintenance to the lens wearer’s ocular environment due to its removing and bactericidal properties.    

Efficacy of worn silicone hydrogel contact lenses desinfectation by a multipurpose solution

http://repositorium.sdum.uminho.pt/bitstream/1822/5529/1/Livia%5b1%5d.pdf

Efficacy of worn silicone hydrogel contact lenses desinfectation by a multipurpose solution

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Universidade do Minho: RepositoriUM