The most common antifungal agents used to treat candidiasis are fluconazol and
amphotericinB. Candidal species adhesion is the first step in oral pathogenesis, therefore it is very important to study the behaviour of the adhesion of yeasts submitted to subinhibitory concentrations of antifungal agents. In this study the adhesion was done with Candida
albicans and Candida dubliniensis. In order to mimetize real conditions the adhesion assays
were performed using a medium with artificial saliva and acrylic as substratum, which is the
material used to produce prosthetic devices. The results showed that in the case of Candida
albicans the adherence decreased in the presence of fluconazol (1/4MIC=0,25μg/ml).
Considering Candida dubliniensis there were no significative differences in the number of
adhered cells to acrylic either in the presence of fluconazol or amphotericinB
(1/4MIC=0,01μg/ml). Observing cell morphology after adhesion, by SEM, it was possible to
notice that cells incubated with subMIC fluconazol formed less hifas than those grown in the
presence of subMIC amphotericinB, which can explain the differences in adhesion
