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Bioprinting Using Dual Injection Multi-dimensional Embedding Of Hydrogels

Abstract

Biological printing (bioprinting) is a process used by tissue engineers to efficiently produce living tissue and organs. Current methods of bioprinting rely on standard three axis 3D printing techniques using free standing printed structures, which limits the ability to print multidimensional soft tissue structures. In order to overcome this limitation we have developed a system capable of printing into a biocompatible, degradable, hydrogel substrate, which acts as the supportive material for biological inks (bioinks). We have also created our own hydrogel based bio-ink, capable of solidifying and maintaining its printed structure within the substrate. Our bioprinter also features a new technique for angular printing, allowing any necessary material to be added to the printed structure from any angle, without disturbing the structure

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