Cancer currently stands as the second-leading cause of death worldwide. Studies reveal colorectal cancer (CRC) to be the 4th leading cause of mortality due to cancer. It is estimated that about 30% of CRC cases are hereditary, of which 5% are attributed by known syndromes, particularly Lynch Syndrome. This pilot study aims to fabricate a DNA-graphene-polypyrrole (DGP) based biosensor to diagnose deficiency of functional MMR proteins present in patients at a scale of less than ng/ ml. We have followed LAB-on-CHIP method. We find that the interactive forces between avidin and graphene are mainly hydrophobic, along with some van der Waals, electrostatic and hydrogen bonding interactions. Different mismatch combinations were performed, to prove the activity of each component on the chip. 30 such combinations were done. Electrochemical impedance spectroscopy was done to confirm the working of the bio sensor by corresponding change in electrical impedance. To assist this real-world system, we have carried out simulation studies as well. In the simulation studies from 0-200ns, we present the progression structures of human MutS protein to biotinylated DNA that has been fixed to simulate the manner of a biosensor, furthermore the mismatch within the DNA has been manually introduced with the aid of computational tools to reveal the interactions of the DNA and the protein. This research additionally permits us in early detection of colorectal cancer and the mapping and expertise of the method related to the area of the mismatch repair
