In this research on creating controlled nanopatterns, a novel technique using electrospinning of iron oxide nanoparticles is being investigated, where nanopatterns of biologically sequestered magnetite are proposed. This is because electrospinning is normally used in the fabrication of nanofibers governed by the electrical forces on the surface of the precursor organic fluids thereby producing polymer filaments using an electrostatic force. This electrospinning technique can serve various purposes such as the fine control of the fiber diameters, the production of a defect-free or defect-controllable fiber surface, and the formation of continuous single nanofibers. The electrospinning products are currently being used in applications such as fabrication of scaffolds in tissue engineering, substrates for culturing numerous biological cellular structures and various biosensing applications