With gastroenteritis as the second leading cause of morbidity and mortality worldwide (Guerrant et al. 2001), it is imperative to have a laboratory method which is highly rapid, sensitive, and cost-effective to aid in the diagnosis and treatment of affected patients. This validation study seeks to evaluate the Nanosphere Verigene real-time PCR and microarray assay as a potential replacement for the current culture-based methods of testing stool samples for enteric pathogens in the Bridgeport Hospital microbiology lab