A poster discussing "shade avoidance" in A. thaliana plants

Burghardt, Liana

Engelmann, Kathleen

Gelgud, Ryan

Larson, Hannah

Lorusso, Amanda

Otfinoski, Jamie

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Genetic map of the Kas-1 x Col-gl1 RIL population p = < 0.05, ** p =  < 0.001, ***p = < 0.0001 Strain x Treatment interactions indicate loci controlling the response to oscillating temperature may differ between strains. Engelmann, Kathleen [1], Larson, Hannah [1, Lorusso, Amanda [1], Gelgud, Ryan [1], Otfinoski, Jamie [1], Burghardt, Liana [2] 1 - University of Bridgeport, Biology, 126 Park Avenue, Bridgeport, CT, 06604, USA 2 - Duke University, Biology, Box 90338, Durham, NC, 27708, USA  Plants normally experience daily temperature fluctuations.  The Treatments  Quantitative trait loci associated with the "shade avoidance" type response in               A. thaliana when grown under oscillating daily summer temperatures. These fluctuation can be as narrow as 5oC, but are often well over 10oC, especially in the summer.  In the lab, A. thaliana rarely experiences fluctuations broader than 2oC.  How does this affect our understanding of the growth, physiology and genetics of Arabidopsis?   Plants were grown in Percival I-360 Incubators programmed to simulate summer field conditions in Norwich, UK, at the southern extreme of the normal range for summer growth of A. thaliana. Control Chamber- Orange line Fluctuating Chamber- Red line  Most Arabidopsis are winter annuals, however, in cooler climates, some strains can grow and flower over the summer. Constant Temperature Oscillating Temperature Growth under oscillating temperatures induces a ‘shade-avoidance’ type response. Plants grow longer thinner, leaves, similar to when they are grown in shade. But- Is this response the same across different strains? The Genes The Traits The Plants NGA590.0446063542.4T1G116.5NGA639.74460731513.64460647619.8MSAT1.123.52160761529.0AthZFPG30.3NGA24833.64460796351.7T27K12-SP655.32160754056.12160706561.74460715265.02160705767.9NGA280 2160692871.62160746374.22160770083.4MSAT1.1389.6NGA69296.82160703099.344606525101.71MSAT2.50.0NGA11457.9446063229.9CIW213.044606142 2160703820.5MSAT2.3823.2CIW330.42160725931.8THY-136.8PHYB42.74460772748.34460791450.7PLS753.2NGA112660.5MSAT2.4162.34460782468.32160715771.9NGA36175.2MSAT2.775.8NGA16878.34460681080.94460792285.94460676988.94460653391.290J19T799.12NGA320.0216074791.9446066078.1446072839.8ATCHIB211.62160717514.14460708719.2Z3081723.04460672145.5GL149.52160749654.3T0410967.24460638779.52160768385.04460633094.6R3002597.4NGA6 44606273101.73MSAT4.390.0CIW56.0446080288.44460662312.0NGA821.74460795528.82160760633.7MSAT4.2536.8MSAT4.1545.12160751348.44460754549.8CD3-6952.2MSAT4.1854.32160739456.32160718459.24460628963.8MSAT4.967.5nga113968.14460668870.2MSAT4.3374.0NGA110777.9MSAT4.3779.44446070460.0446072347.7NGA22511.6NGA24921.02160740222.94460615925.34460724227.84460616729.6MSAT5.1432.24460669633.5NGA13935.82160714839.74460766844.8MSAT5.2249.94460633852.6MI13755.94460645267.4CIW968.8MSAT5.969.54460634670.74460776773.04460780877.3NGA12980.8MSAT5.1287.34460799593.54460650994.64460741399.7LFY3102.4M555111.05Mechanisms of Sustainable reDevelopment: Lessons from Plants, Justin Borevitz   I                II             III                    IV               V Parental Lines:  Kashmir (A wild strain from the Himalayan region)     Requires vernalization (cold exposure prior to flowering) Columbia-gl1 (Derived from Columbia-1,a lab strain)     Does not require vernalization  Known to segregate for several growth and flowering genes such as FRI, FLC, FLM, MAF2, CRY2, and HUA2   92 Kas-1 x Col-gl1 Recombinant Inbred Lines Vegetative Traits Strain Trt SxT Control Oscillating 2 pair true leaves: Early Leaf Length *** *** I(t)             V(2) none Early Petiole Length * ***             IV  V(2)             IV Early Width * * I(b)            V(t)                 V(2) 2  4 Leaves (days)                   V(b) none 4 pair true leaves: Late Leaf Length * *** I none Late Petiole Length ** *** I I Late Width I  II  III          III 4  8 Leaves (days) **                   V (t)     II Reproductive Traits Onset of Flowering Leaf Length I           IV V(t) none Petiole Length I (2)      IV         none Width                  V(t)             IV V(t)  2 Leaves F.  (days) I (2)      IV V(t) I           IV Leaf # I           IV V(t) none Rosette size I           IV none 1st Dehiscent Fruit Flower Height     II      IV none # Fruit                  V(m) I         IV V(m) Mean Angle (overall) I           IV none Loci detected by Composite Interval Mapping  Loci detected by ANOVA*  QTL: Control QTL: Oscillating   I    II  III        IV       V QTL Maps also show that different loci are detected under oscillating conditions! No ANOVAs: Kas-1 does not flower without vernalization! But it does under oscillating temperaures! Under lab conditions, we detect several QTL containing ‘known’ growth and flowering genes. However, many of these loci cannot be detected under oscillating summer temperatures! We measured expression levels of 6 known growth and flowering genes located in our QTL using RT-PCR. Four showed strain-specific differences in the levels of expression under oscillating summer temperatures. # 3 :  __  __  __ 

Quantitative Trait Loci Associated with the "Shade Avoidance" Type Response in A. thaliana when Grown under Oscillating Daily Summer Temperatures

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Quantitative Trait Loci Associated with the "Shade Avoidance" Type Response in A. thaliana when Grown under Oscillating Daily Summer Temperatures

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