Amanda Scott, Bryant Brown, John Solomon's poster about Arabidopsis thaliana seeds and there ability to survive exposure to extreme cold

Brown, Bryant

Engelmann, Kathleen

Scott, Amanda

Solomon, John

UB ScholarWorks

Hypothesis Can Arabidopsis thaliana Seeds Survive Exposure to Extreme Cold? Amanda Scott, Bryant Brown, John Solomon Biology Program Advised By: Dr. Engelmann College of Arts and Science University of Bridgeport Bridgeport, CT The Arabidopsis thaliana Columbia (Col-0) and will not germinate after exposure to liquid nitrogen from one minute and up resulting in no growth. The Arabidopsis thaliana that are least exposed to liquid nitrogen and 24 hours (-4°C) in the freezer, will grow. Conclusion The best growth was in the 24 hour treatment. Slight exposure to liquid nitrogen may cause acceleration in growth in the Columbia strain. The acceleration in growth could be due to the cells ability to want to grow more rapid once exposed to extremely frigid temperatures. The Columbia strain can tolerate the extreme cold temperatures of liquid nitrogen and general cold temperature. Length of the Seedling Vs. Exposure Time              Late/Longest showing statistically significant in            Results Background  Arabidopsis thaliana is a small flowering plant, from the Mustard (Brassicaceae) family. The mustard (Brassicaceae) family includes cultivated species such as cabbage and radish. Arabidopsis thaliana have a rapid life cycle (6 weeks from germination to mature seeds) and is native to Europe and Asia.   A. thaliana has a winter annual life cycle. Seedlings germinates in  fall or late summer, over winter as rosettes, and flower in early spring or summer).   Depending on the type of plant, the affects of cold temperature varies. Some plants, like A. thaliana, need a period moist cold temperature before germination.   A. thaliana  was chooses specifically because it is tolerant, even dependent, on cold environmental temperatures.  Liquid nitrogen is very cold. At normal atmospheric pressure it’s between  -196 to -210, substantially below the normal winter temperatures experienced by A . thaliana seeds.      Method Arabidopsis thaliana seeds (Columbia strain) were exposed to 5 different treatments. The Columbia strain was used because of its resistance to cold temperature, making it the perfect strain for this experiment. The treatment includes the control with no exposure, 24 hours in the freezer (-4°C), 3 sec to liquid nitrogen, 1 min to liquid nitrogen and 1 hour to liquid nitrogen, after stratification. The growth treatment was in agar plates called UltraSpec-AgaroseTM Gel. Each plate was separated in half. In both halves, there were approximately 8-10 seeds each from both strains in the same medium, with the same exposure time (treatment), with the plates labeling Control, 24 hours, 3 sec, 1 min, and 1 hour. The plants grew in a 20°C temperature and 18°C at night. Plants was exposed to 16 hours of light and 8 hours of dark. After germination the overall length of each plant was recorded daily for 8 days. The longest and the shortest plant measured was recorded, the average of the two data recorded was considered the overall growth of the Col-0 strain within its specific treatment. 00.10.20.30.40.50.60.7Day 1 Day 2 Day 3 Day 4 Day 5 Day 6 Day 7 Day 8Growth in Length Days Columbia Strain Daily Growth  Control (cm) Col3 Seconds (cm) Col1 Minutes (cm) Col1 Hour (cm) Col24 Hours (cm) Col00.10.20.30.40.50.60.7Col Col Col Col ColControl (cm) 3 Seconds (cm) 1 Minutes (cm) 1 Hour (cm) 24 Hours (cm)Growth  in Length Columbia Strain Treatments Columbia Strain Daily Growth  Day 1Day 2Day 3Day 4Day 5Day 6Day 7Day 8Acknowledgements: Funded by UB Biology Department, and Fellow Lab members Rsquare : 0.13429 Prob > F : 0.0200*  Shows the Columbia Strain’s growth between days 5-8 as exposed to liquid nitrogen. There is statistic significant increase in seedling length with increasing exposure time.  A.S. A.S. B.B. B.B. http://network.nature.com/system/group/000/000/325/original.png  

Can Arabidopsis thaliana Seeds Survive Exposure to Extreme Cold?

https://scholarworks.bridgeport.edu/xmlui/bitstream/handle/123456789/310/Faculty%20Research%20Day%20Poster%202014%20SSB%20KE.pdf?sequence=3&isAllowed=y

Can Arabidopsis thaliana Seeds Survive Exposure to Extreme Cold?

Abstract

Similar works

Full text

Available Versions

UB ScholarWorks