Détermination sur tissu endométrial néoplasique de profils d'expression de micro-ARNs associés à l'envahissement ganglionnaire dans le cancer de l'endomètre de type endométrioïde de stade précoce

Abstract

Current histological classification of early-stage endometrial cancer (EC) may show insufficient accuracy to precisely predict lymph node metastases leading to potential over or under treatment. Hence, additional highly sensitive and specific molecular prognostic biomarkers correlated with prognostic factors, such as nodal involvement and lymphovascular space involvement (LVSI), are needed to better adapt surgical management and adjuvant therapies. We fstudied by microarray analysis microRNA expression profiles of formalin-fixed paraffin-embedded grade 1–2 supposed early-stage endometrioid adenocarcinomas specimen. The expression levels of 5 microRNAs (miR-34c-5p, -375, -184, -34c-3p, et -34b-5p) were lower in the EC with positive nodal status compared to those with negative nodal status. The expression levels of 3 microRNAs (miR-34c-5p, -23b-5p, et -23c) were lower in the EC with positive LVSI compared to those with negative LVSI. A quantitative reverse transcriptase–PCR assay was used to determine micro-RNAs thresholds correlated with prognostic factors. Women with a microRNA-184-fold change 0.30 (n=3; 11.5%), p=0.006. Women with a microRNA-34c-5p fold change 0.15 (n=0; 0.0%), p0,30, p=0,006. Les patientes avec seuil d'expression du micro-ARN-34c-5p 0,15 (0,0%), p<0,001. Ces profils d'expression de micro-ARNs fournissent les bases pour de nouvelles études sur la fonction des micro-ARNs dans le cancer de l'endomètre et pourraient constituer un nouvel outil au diagnostic du statut ganglionnaire