Metabolomics of 2-OG treated LN18 IDH1 mutant and wild-type cells

Abstract

These data were produced from the analysis of LN18 cells with (MUT) and without (WT) a transduced isocitrate dehydrogenase 1 (IDH1) mutation, treated with 10 mM 2-oxoglutarate (2-OG) or vehicle control for 24 hours. The data were acquired as part of the project “Investigating Isocitrate Dehydrogenase Mutations in Cancer Using New Metabolomics Methods” using multiple liquid chromatography-mass spectrometry methods. Anion-exchange chromatography-mass spectrometry (IC-MS) data were acquired on a Dionex IC-5000+ ion chromatography system hyphenated to a Thermo Fisher Scientific Exactive. Reversed-phase chromatography-mass spectrometry (RP-MS) data were acquired on a Dionex U3000 UHPLC system hyphenated to a Thermo Fisher Scientific Exactive. Derivatised RP-MS data were acquired on a Dionex U3000 UHPLC system hyphenated to a Thermo Fisher Scientific Exactive with sample derivatisation using 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate. Data are .zip compressed to reduce file size. Data files in the .RAW format require Thermo Fisher Scientific Xcalibur software to analyse