Background. The modalities of malaria transmission along the Thailand-Myanmar border are poorly understood. Here we address the relevance of using a specific Anopheles salivary biomarker to measure the risk of human exposure to anopheles bites. Methods. Serologic surveys were conducted from May 2013 to December 2014 in 4 sentinel villages. Dried blood spots (n>9,400) were collected in filter papers among all inhabitants at baseline and then every three months up to 18 months. The relationship between the intensity of human antibody response and entomological indicators of transmission (Human biting rates and Entomological Inoculation rates or EIR) was studied using a multivariate three-level mixed model analysis. “Heatmaps” for human IgG responses for each village and survey time point were created using QGIS 2.4. Results. The levels of IgG response of participants significantly varied according to the village, season and age (P<0.001) and were positively associated with the abundance of total Anopheles spp. and primary malaria vectors and EIR (P<0.001). Spatial clusters of high IgG responders were identified across space and time within study villages. Conclusions. The gSG6-P1 biomarker has great potential to address the risk of transmission along the Thailand-Myanmar border and represents a promising tool to guide malaria intervention
