Banerjee, Abhik

Chong, Johnny Gan

Chou, Tom

Dror, Iris

Guttman, Mitchell

Jacobson, Elsie C.

Luong, Christy

Maestrini, Davide

Markaki, Yolanda

Mistry, Bhaven A.

Plath, Kathrin

Schöneberg, Johannes

Tan, Shawn Y. X.

Wang, Yuying

Balai Penelitian Tanaman Kacang-kacangan dan Umbi-umbian

lib.bppsdmp.pertanian.go.id

Hasil utama penelitian kacang-kacangan dan umbi-umbian tahun 2005

The long non-coding RNA Xist exploits numerous effector proteins to progressively induce gene silencing across the X chromosome and form the inactive X (Xi)-compartment. The mechanism underlying formation of the chromosome-wide Xi-compartment is poorly understood. Here, we find that formation of the Xi-compartment is induced by ~50 locally confined granules, where two Xist RNA molecules nucleate supra-molecular complexes (SMCs) of interacting proteins. Xist-SMCs are transient structures that concentrate rapidly recycling proteins in the X by increasing protein binding affinity. We find that gene silencing originates at Xist-SMCs and propagates across the entire chromosome over time, achieved by Polycomb-mediated coalescence of chromatin regions and aggregation, via its intrinsically disordered domains, of the critical silencing factor SPEN. Our results suggest a new model for X chromosome inactivation, in which Xist RNA induces macromolecular crowding of heterochromatinizing proteins near distinct sites which ultimately increases their density throughout the chromosome. This mechanism enables deterministic gene silencing without the need for Xist ribonucleoprotein complex-chromatin interactions at each target gene

Caltech Authors - Main

English

Xist-seeded nucleation sites form local concentration gradients of silencing proteins to inactivate the X-chromosome

https://authors.library.caltech.edu/106787/1/2020.11.22.393546v1.full.pdf

Hasil utama penelitian kacang-kacangan dan umbi-umbian tahun 2005

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lib.bppsdmp.pertanian.go.id

Caltech Authors - Main