10.1371/journal.pone.0082259PLoS ONE812-POLN

A Stamatakis

Ajith Nagahawatte

AM Powers

Angelia Chow

B Pastorino

C Frank

Champica Bodinayake

Christopher W. Woods

D Nkoghe

D Silvestro

D Taraphdar

DJ Gubler

DR Munasinghe

Duane J. Gubler

FigTree

FJ Burt

G Borgherini

G Pialoux

G Rezza

HA Hapuarachchi

HC Hapuarachchi

I Schuffenecker

JE Staples

John J. Strouse

JT Hertz

K Katoh

K Laoprasopwattana

KA Tsetsarkin

KB Gibney

Lark L. Coffey

M Panning

ME Reller

Megan E. Reller

NP Kumar

October M. Sessions

P Hochedez

P-Y Shu

Robert Chua

S Nimmannitya

SA Kularatne

SD Blacksell

SD Thiberville

Truls Østbye

Ufuoma Akoroda

Vasantha Devasiri

Wasantha Kodikaarachchi

Y Apandi

Yan'an Hou

PubMed

Chikungunya virus (CHIKV) re-emerged in Sri Lanka in late 2006 after a 40-year hiatus. We sought to identify and characterize acute chikungunya infection (CHIK) in patients presenting with acute undifferentiated febrile illness in unstudied rural and semi-urban southern Sri Lanka in 2007. We enrolled febrile patients ≥ 2 years of age, collected uniform epidemiologic and clinical data, and obtained serum samples for serology, virus isolation, and real-time reverse-transcriptase PCR (RT-PCR). Serology on paired acute and convalescent samples identified acute chikungunya infection in 3.5% (28/797) patients without acute dengue virus (DENV) infection, 64.3% (18/28) of which were confirmed by viral isolation and/or real-time RT-PCR. No CHIKV/DENV co-infections were detected among 54 patients with confirmed acute DENV. Sequencing of the E1 coding region of six temporally distinct CHIKV isolates (April through October 2007) showed that all isolates posessed the E1-226A residue and were most closely related to Sri Lankan and Indian isolates from the same time period. Except for more frequent and persistent musculoskeletal symptoms, acute chikungunya infections mimicked DENV and other acute febrile illnesses. Only 12/797 (1.5%) patients had serological evidence of past chikungunya infection. Our findings suggest CHIKV is a prominent cause of non-specific acute febrile illness in southern Sri Lanka.

Reller Megan E.

Akoroda Ufuoma

Nagahawatte Ajith

Devasiri Vasantha

Kodikaarachchi Wasantha

Strouse John J.

Chua Robert

Hou Yan'an

Chow Angelia

Sessions October M.

Østbye Truls

Gubler Duane J.

Woods Christopher W.

Bodinayake Champica

Public Library of Science (PLOS)

Chikungunya as a Cause of Acute Febrile Illness in Southern Sri Lanka

BACKGROUND: Chikungunya virus (CHIKV) re-emerged in Sri Lanka in late 2006 after a 40-year hiatus. We sought to identify and characterize acute chikungunya infection (CHIK) in patients presenting with acute undifferentiated febrile illness in unstudied rural and semi-urban southern Sri Lanka in 2007. METHODOLOGY/PRINCIPAL FINDINGS: We enrolled febrile patients ≥ 2 years of age, collected uniform epidemiologic and clinical data, and obtained serum samples for serology, virus isolation, and real-time reverse-transcriptase PCR (RT-PCR). Serology on paired acute and convalescent samples identified acute chikungunya infection in 3.5% (28/797) patients without acute dengue virus (DENV) infection, 64.3% (18/28) of which were confirmed by viral isolation and/or real-time RT-PCR. No CHIKV/DENV co-infections were detected among 54 patients with confirmed acute DENV. Sequencing of the E1 coding region of six temporally distinct CHIKV isolates (April through October 2007) showed that all isolates posessed the E1-226A residue and were most closely related to Sri Lankan and Indian isolates from the same time period. Except for more frequent and persistent musculoskeletal symptoms, acute chikungunya infections mimicked DENV and other acute febrile illnesses. Only 12/797 (1.5%) patients had serological evidence of past chikungunya infection. CONCLUSIONS/SIGNIFICANCE: Our findings suggest CHIKV is a prominent cause of non-specific acute febrile illness in southern Sri Lanka

Reller, Megan E

Akoroda, Ufuoma

Nagahawatte, Ajith

Devasiri, Vasantha

Kodikaarachchi, Wasantha

Strouse, John J

Chua, Robert

Hou, Yan'an

Chow, Angelia

Sessions, October M

Østbye, Truls

Gubler, Duane J

Woods, Christopher W

Bodinayake, Champica

DukeSpace (Duke Univ.)

PLoS ONE

Chikungunya as a cause of acute febrile illness in southern Sri Lanka.

<div>BackgroundChikungunya virus (CHIKV) re-emerged in Sri Lanka in late 2006 after a 40-year hiatus. We sought to identify and characterize acute chikungunya infection (CHIK) in patients presenting with acute undifferentiated febrile illness in unstudied rural and semi-urban southern Sri Lanka in 2007. Methodology/Principal FindingsWe enrolled febrile patients ≥ 2 years of age, collected uniform epidemiologic and clinical data, and obtained serum samples for serology, virus isolation, and real-time reverse-transcriptase PCR (RT-PCR). Serology on paired acute and convalescent samples identified acute chikungunya infection in 3.5% (28/797) patients without acute dengue virus (DENV) infection, 64.3% (18/28) of which were confirmed by viral isolation and/or real-time RT-PCR. No CHIKV/DENV co-infections were detected among 54 patients with confirmed acute DENV. Sequencing of the E1 coding region of six temporally distinct CHIKV isolates (April through October 2007) showed that all isolates posessed the E1-226A residue and were most closely related to Sri Lankan and Indian isolates from the same time period. Except for more frequent and persistent musculoskeletal symptoms, acute chikungunya infections mimicked DENV and other acute febrile illnesses. Only 12/797 (1.5%) patients had serological evidence of past chikungunya infection. Conclusions/SignificanceOur findings suggest CHIKV is a prominent cause of non-specific acute febrile illness in southern Sri Lanka. </div

Megan E. Reller (491579)

Ufuoma Akoroda (491580)

Ajith Nagahawatte (491581)

Vasantha Devasiri (491582)

Wasantha Kodikaarachchi (491583)

John J. Strouse (491584)

Robert Chua (243441)

Yan'an Hou (491585)

Angelia Chow (350499)

October M. Sessions (389415)

Truls Østbye (491586)

Duane J. Gubler (248346)

Christopher W. Woods (104194)

Champica Bodinayake (491587)

The Francis Crick Institute

Chikungunya virus (CHIKV) re-emerged in Sri Lanka in late 2006 after a 40-year hiatus. We sought to identify and characterize acute chikungunya infection (CHIK) in patients presenting with acute undifferentiated febrile illness in unstudied rural and semi-urban southern Sri Lanka in 2007.We enrolled febrile patients ≥ 2 years of age, collected uniform epidemiologic and clinical data, and obtained serum samples for serology, virus isolation, and real-time reverse-transcriptase PCR (RT-PCR). Serology on paired acute and convalescent samples identified acute chikungunya infection in 3.5% (28/797) patients without acute dengue virus (DENV) infection, 64.3% (18/28) of which were confirmed by viral isolation and/or real-time RT-PCR. No CHIKV/DENV co-infections were detected among 54 patients with confirmed acute DENV. Sequencing of the E1 coding region of six temporally distinct CHIKV isolates (April through October 2007) showed that all isolates posessed the E1-226A residue and were most closely related to Sri Lankan and Indian isolates from the same time period. Except for more frequent and persistent musculoskeletal symptoms, acute chikungunya infections mimicked DENV and other acute febrile illnesses. Only 12/797 (1.5%) patients had serological evidence of past chikungunya infection.Our findings suggest CHIKV is a prominent cause of non-specific acute febrile illness in southern Sri Lanka

Megan E Reller

John J Strouse

October M Sessions

Duane J Gubler

Christopher W Woods

Directory of Open Access Journals

Crossref

http://scholarbank.nus.edu.sg/bitstream/10635/110503/1/2013-chikungunya_cause_acute_febrile_illness-published.pdf

Chikungunya as a cause of acute febrile illness in southern Sri Lanka

Chikungunya as a cause of acute febrile illness in southern Sri Lanka

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Public Library of Science (PLOS)

DukeSpace (Duke Univ.)

Public Library of Science (PLOS)

The Francis Crick Institute

Directory of Open Access Journals

Crossref