Alicia Rodriguez-Pla

Alisa Gotte

Anna Karolina Palucka

Chavan

Gabay

Holden T. Maecker

Jacques Banchereau

Jeanine Baisch

Jose Rossello-Urgell

Kaneko

Lorien Nassi

Lynda Bennett

Marilynn Punaro

Nicole Baldwin

Pinakeen Patel

Schlitt

Steinberger

Tracey Wright

Vallin

Victoria Cantrell

Virginia Pascual

Abstract
               Blood monocytes from children with systemic lupus erythematosus (SLE) behave similar to dendritic cells (DCs), and SLE serum induces healthy monocytes to differentiate into DCs in a type I IFN–dependent manner. In this study, we found that these monocytes display significant transcriptional changes, including a prominent IFN signature, compared with healthy controls. Few of those changes, however, explain DC function. Exposure to allogeneic T cells in vitro reprograms SLE monocytes to acquire DC phenotype and function, and this correlates with both IFN-inducible (IP10) and proinflammatory cytokine (IL-1β and IL6) expression. Furthermore, we found that both IFN and SLE serum induce the upregulation of CCR7 transcription in these cells. CCR7 protein expression, however, requires a second signal provided by TLR agonists such as LPS. Thus, SLE serum “primes” a subset of monocytes to readily (&amp;lt;24 h) respond to TLR agonists and acquire migratory DC properties. Our findings might explain how microbial infections exacerbate lupus.</jats:p

Crossref

The Journal of Immunology

IFN Priming Is Necessary but Not Sufficient To Turn on a Migratory Dendritic Cell Program in Lupus Monocytes

IFN Priming Is Necessary but Not Sufficient To Turn on a Migratory Dendritic Cell Program in Lupus Monocytes

Abstract

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