Babesia and Theileria spp. are tick transmitted protozoa parasites affecting various animals worldwide. Theileria cervi affects the white tailed deer populations of the United States, particularly fawns, and Babesia sp. coco was recently described and affects dogs in the United States. Ticks were collected from 15 sites in the Oklahoma City area and screened using polymerase chain reaction (PCR) assays. T. cervi, B. sp. coco, and a possible Babesia sp. were detected in the ticks, and a notable difference in prevalence rates was present in 3 sites. The presence of these pathogens suggests that their natural hosts are also present in the area, though more investigation is required to determine the source of these populations