10.1371/journal.pone.0096661PLoS ONE95-POLN

AH Fong

B Gupta

B Povazay

Ching-Yu Cheng

EA Maul

Elizabeth Sidhartha

F Gomi

G Yiu

H Koizumi

HE Grossniklaus

J Ho

J Tian

Jean Martial Mari

JL Fleiss

JM Bland

JM Mari

JW Shin

L Duan

L Zhang

M Rosman

ME Fitzgerald

Michael J. A. Girard

MJ Girard

MR Hee

N Foin

P Lin

Preeti Gupta

RA Linsenmeier

RF Spaide

SE Chung

T Fujiwara

T Torzicky

T Yamashita

Tien-Yin Wong

Y Ikuno

Y Imamura

Yingfeng Zheng

PubMed

To evaluate a simplified method to measure choroidal thickness (CT) using commercially available enhanced depth imaging (EDI) spectral domain optical coherence tomography (SD-OCT).We measured CT in 31 subjects without ocular diseases using Spectralis EDI SD-OCT. The choroid-scleral interface of the acquired images was first enhanced using a post-processing compensation algorithm. The enhanced images were then analysed using Photoshop. Two graders independently graded the images to assess inter-grader reliability. One grader re-graded the images after 2 weeks to determine intra-grader reliability. Statistical analysis was performed using intra-class correlation coefficient (ICC) and Bland-Altman plot analyses.Using adaptive compensation both the intra-grader reliability (ICC: 0.95 to 0.97) and inter-grader reliability (ICC: 0.93 to 0.97) were perfect for all five locations of CT. However, with the conventional technique of manual CT measurements using built-in callipers provided with the Heidelberg explorer software, the intra- (ICC: 0.87 to 0.94) and inter-grader reliability (ICC: 0.90 to 0.93) for all the measured locations is lower. Using adaptive compensation, the mean differences (95% limits of agreement) for intra- and inter-grader sub-foveal CT measurements were −1.3 (−3.33 to 30.8) µm and −1.2 (−36.6 to 34.2) µm, respectively.The measurement of CT obtained from EDI SD-OCT using our simplified method was highly reliable and efficient. Our method is an easy and practical approach to improve the quality of choroidal images and the precision of CT measurement

Gupta Preeti

Sidhartha Elizabeth

Girard Michael J. A.

Mari Jean Martial

Wong Tien-Yin

Cheng Ching-Yu

Public Library of Science (PLOS)

A Simplified Method to Measure Choroidal Thickness Using Adaptive Compensation in Enhanced Depth Imaging Optical Coherence Tomography

<div>PurposeTo evaluate a simplified method to measure choroidal thickness (CT) using commercially available enhanced depth imaging (EDI) spectral domain optical coherence tomography (SD-OCT).MethodsWe measured CT in 31 subjects without ocular diseases using Spectralis EDI SD-OCT. The choroid-scleral interface of the acquired images was first enhanced using a post-processing compensation algorithm. The enhanced images were then analysed using Photoshop. Two graders independently graded the images to assess inter-grader reliability. One grader re-graded the images after 2 weeks to determine intra-grader reliability. Statistical analysis was performed using intra-class correlation coefficient (ICC) and Bland-Altman plot analyses.ResultsUsing adaptive compensation both the intra-grader reliability (ICC: 0.95 to 0.97) and inter-grader reliability (ICC: 0.93 to 0.97) were perfect for all five locations of CT. However, with the conventional technique of manual CT measurements using built-in callipers provided with the Heidelberg explorer software, the intra- (ICC: 0.87 to 0.94) and inter-grader reliability (ICC: 0.90 to 0.93) for all the measured locations is lower. Using adaptive compensation, the mean differences (95% limits of agreement) for intra- and inter-grader sub-foveal CT measurements were −1.3 (−3.33 to 30.8) µm and −1.2 (−36.6 to 34.2) µm, respectively.ConclusionsThe measurement of CT obtained from EDI SD-OCT using our simplified method was highly reliable and efficient. Our method is an easy and practical approach to improve the quality of choroidal images and the precision of CT measurement.</div

Preeti Gupta (709811)

Elizabeth Sidhartha (559387)

Michael J. A. Girard (559388)

Jean Martial Mari (559389)

Tien-Yin Wong (159436)

Ching-Yu Cheng (159319)

The Francis Crick Institute

Gupta, Preeti

Sidhartha, Elizabeth

Girard, Michaël, J A

Mari, Jean Martial

Wong, Tien-Yin

Cheng, Ching-Yu

HAL: Hyper Article en Ligne

PURPOSE: To evaluate a simplified method to measure choroidal thickness (CT) using commercially available enhanced depth imaging (EDI) spectral domain optical coherence tomography (SD-OCT). METHODS: We measured CT in 31 subjects without ocular diseases using Spectralis EDI SD-OCT. The choroid-scleral interface of the acquired images was first enhanced using a post-processing compensation algorithm. The enhanced images were then analysed using Photoshop. Two graders independently graded the images to assess inter-grader reliability. One grader re-graded the images after 2 weeks to determine intra-grader reliability. Statistical analysis was performed using intra-class correlation coefficient (ICC) and Bland-Altman plot analyses. RESULTS: Using adaptive compensation both the intra-grader reliability (ICC: 0.95 to 0.97) and inter-grader reliability (ICC: 0.93 to 0.97) were perfect for all five locations of CT. However, with the conventional technique of manual CT measurements using built-in callipers provided with the Heidelberg explorer software, the intra- (ICC: 0.87 to 0.94) and inter-grader reliability (ICC: 0.90 to 0.93) for all the measured locations is lower. Using adaptive compensation, the mean differences (95% limits of agreement) for intra- and inter-grader sub-foveal CT measurements were -1.3 (-3.33 to 30.8) µm and -1.2 (-36.6 to 34.2) µm, respectively. CONCLUSIONS: The measurement of CT obtained from EDI SD-OCT using our simplified method was highly reliable and efficient. Our method is an easy and practical approach to improve the quality of choroidal images and the precision of CT measurement

Gupta, P

Sidhartha, E

Girard, MJA

Mari, JM

Wong, TY

Cheng, CY

University of Melbourne Institutional Repository

PLoS ONE

A simplified method to measure choroidal thickness using adaptive compensation in enhanced depth imaging optical coherence tomography

Crossref

Michael J A Girard

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A simplified method to measure choroidal thickness using adaptive compensation in enhanced depth imaging optical coherence tomography.

A simplified method to measure choroidal thickness using adaptive compensation in enhanced depth imaging optical coherence tomography

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Public Library of Science (PLOS)

The Francis Crick Institute

HAL: Hyper Article en Ligne

University of Melbourne Institutional Repository

Crossref

Directory of Open Access Journals

Public Library of Science (PLOS)