Nicotine, readily available in electronic nicotine delivery systems, poses a lethal threat as it is easily accessible and highly toxic in its liquid form. Seventy-five percent of nicotine is metabolized into cotinine, and with the growing prevalence of nicotine-related deaths comes the increased possibility of finding nicotine or cotinine in the tissues of a corpse and thus the possibility of distorting the postmortem interval (PMI). Through entomotoxicology, the study of how drugs and toxins influence the development of insects present on a decomposing body, this experiment aimed to determine if varying levels of nicotine and cotinine affect the development of the forensically important Cochliomyia macellaria (Fabricius) [Diptera: Calliphoridae]. In this experiment, C. macellaria maggots were reared on seven varying concentrations of nicotine and cotinine and were sampled every eight hours. Length, weight and accumulated degree hours were then measured or determined. Our study found that maggots reared on lethal nicotine and lethal cotinine showed a significant decrease in length and weight as well as a delay in development time for the second instar life stage. These results suggest a relationship between high concentrations of nicotine and cotinine and the development of C. macellaria, which could greatly affect PMI estimations.Biography: Elise Hodges, Tessa Fungo, Gautham, and Hannah Bowlin are undergraduate honor’s students who conducted a research project in the field of forensic entomology as a part of Dr. Heather Ketchum’s perspectives course, “Bugs, Thugs, and Science” in fall 2018.University Libraries Undergraduate Research Awardundergraduat
