B Oyungerel

BR Yun

DW Choi

IJ Reynolds

J Liu

JB Weon

JD Ly

M Ankarcrona

M Duchen

M Fukui

M Koga

MR Hynd

P Albrecht

RD Randall

RH Swerdlow

S Tan

SS Lim

TH Murphy

WR Markesbery

Y Shen

AbstractMagnoliae Flos (MF) is a traditional medicinal herb used for managing rhinitis, sinusitis and headache. The purpose of the present study was to determine the neuroprotective effect of MF against glutamate-induced oxidative stress and to assess the underlying mechanism. Glutamate is a major endogenous excitatory neurotransmitter in the brain and contributes to the development of neurodegenerative diseases by excessive activation. MF extract was subjected to a neuroprotective effect assay in HT22 mouse hippocampal cells. The mechanism underlying the neuroprotective effect of MF extract was evaluated by assaying reactive oxygen species (ROS) levels, intracellular Ca2+ levels, mitochondrial membrane potential, glutathione level and antioxidant enzyme activity in HT22 cells. MF extract significantly decreased glutamate-induced death of HT22 cells (80.83 ± 7.34% relative neuroprotection). MF extract reduced the intracellular ROS and Ca2+ levels and increased the glutathione level and glutathione reductase and glutathione peroxide activities. Moreover, MF extract attenuated the mitochondrial membrane potential in HT22 cells. These results suggested that MF extract exerts a neuroprotective effect against oxidative stress HT22 cells, which was mediated by its antioxidant activity.</jats:p

Youn Sik Jung

Jin Bae Weon

Woo Seung Yang

Gahee Ryu

Choong Je Ma

Crossref

Scientific Reports

Neuroprotective effects of Magnoliae Flos extract in mouse hippocampal neuronal cells

Neuroprotective effects of Magnoliae Flos extract in mouse hippocampal neuronal cells

Abstract

Similar works

Full text

Available Versions

Crossref