Binley, J.

Crooks, E. T.

Lapedes, A.

Pantophlet, R.

Phung, P.

Taylor, N.

Wrin, T.

Simon Fraser University Institutional Repository

BioMed CentralPage 1 of 1(page number not for citation purposes)RetrovirologyOpen AccessPoster presentationP04-03. Cross-clade neutralization analysis of plasmas from clade B, C and CRF01_AE HIV-infected donorsJ Binley*1, T Wrin2, R Pantophlet3, P Phung2, ET Crooks1, A Lapedes4, N Taylor5, L Cavacini6, G Steigler7, R Kunert7, H Katinger7, C Petropoulos2, D Richman8, L Morris5, R Sutthent9 and DR Burton10Address: 1AIDS/Viral Immunology, Torrey Pines Institute, San Diego, CA, USA, 2MonoGramBio, San Francisco, CA, USA, 3Simon Fraser University, Vancouver, Canada, 4Los Alamos National Laboratory, Los Alamos, NM, USA, 5National Institute for Communicable Diseases, Johannesburg, South Africa, 6Beth Israel Medical Center, Boston, MA, USA, 7University of Agricultural Sciences, Vienna, Austria, 8University of California, San Diego, La Jolla, CA, USA, 9HIV Bioinformatic Center, Bangkok, Thailand and 10The Scripps Research Institute, La Jolla, CA, USA* Corresponding author    BackgroundThe genetic diversity of HIV-1, particularly in Env, is con-sidered a major challenge for vaccine researchers, espe-cially those attempting to elicit broadly neutralizingantibodies. Categorizing HIV strains into neutralizationserotypes may help determine the requirements for maxi-mal vaccine immune coverage. HIV is divided into clades,based on genetic relatedness, principally in Env. Investiga-tions as to whether these clades might help to define neu-tralizing serotypes have so far mostly been confined tosmall studies, and have been hampered by the challengesof traditional PBMC-blast neutralization assays. Theobservation that some HIV+ plasmas neutralize virusesfrom several major clades hints of an undercurrent of con-served neutralization, but its potency relative to any clade-restricted activity is unknown. High throughput pseudovi-rus neutralization assays provide a way to investigate.MethodsHere, we measured neutralization of 45 primary virusesfrom clades B, C and CRF_01 AE by 12 broad plasmasfrom the same 3 clades.ResultsSignificant cross-neutralization was observed, and well-defined neutralizing serotypes was absent. However, eachplasma exhibited a unique pattern against the spectrum ofviruses. Increased intraclade neutralization titers wasobserved in many cases. These were most pronounced forclade B-E viruses that were neutralized 2–10 fold morepotently by clade-matched plasmas in several cases. Wealso investigated binding serotypes by gp120 ELISA,where we observed greater intraclade binding titers for B-E plasmas, mirroring the intraclade neutralization. Map-ping analyses suggested that in some cases, the cross-neu-tralizing activity is mostly directed to gp120 and overlapsthe CD4 binding site.ConclusionThe generally broad neutralization observed suggests thatan immunogen based on one or a few Envs could, in prin-ciple, provide worldwide HIV-1 protection. Clade C Envsmay be worth special attention, considering the particu-larly broad plasma neutralizing activities. The challengeremains to better define the cross-neutralizing specificitiesand moreover, to find immunogens able to induce them.from AIDS Vaccine 2009Paris, France. 19–22 October 2009Published: 22 October 2009Retrovirology 2009, 6(Suppl 3):P31 doi:10.1186/1742-4690-6-S3-P31<supplement> <title> <p>AIDS Vaccine 2009</p> </title> <editor>Anna Laura Ross</editor> <note>Meeting abstracts – A single PDF containing all abstracts in this Supplement is available <a href="http://www.biomedcentral.com/content/files/pdf/1742-4690-6-S3-full.pdf">here</a>.</note> <url>http://www.biomedcentral.com/content/pdf/1471-2105-10-S12-info.pdf</url> </supplement>This abstract is available from: http://www.retrovirology.com/content/6/S3/P31© 2009 Binley et al; licensee BioMed Central Ltd. 

P04-03. Cross-Clade Neutralization Analysis of Plasmas from Clade B, C and Crf01_Ae Hiv-Infected Donors

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