A Gutierrez-Fernandez

A Jenner

A Kmin

A Shukla

AD Agaiby

AE Loo

AL Brubaker

Alvin Eng Kiat Loo

AM Rasik

AM Tester

B Halliwell

Barry Halliwell

BC Nwomeh

CY Lee

D Tang

EJ Miller

G Murphy

I Goren

J Goldner

JD Morrow

JS Beckman

Juan Sastre

JV Dovi

KA Hasty

LL Bennett

LR Prince

M Schafer

Martin Wasser

MB Benhanifia

MJ Reiss

N Nishio

N Ojha

NJ Trengove

P Niethammer

PH Kwakman

PL Danielsen

R Hanemaaijer

R Lobmann

R Moseley

Rongjian Ho

S Rieger

S Roy

S Schreml

S Yeoh-Ellerton

SK Yoo

T Nomura

Tiehua Du

TR Kyriakides

TZ Kirk

Wee Thong Ng

X Wang

Y Fang

Yee Ting Wong

YJ Suzuki

PLoS ONE

PubMed

It has been established that low concentrations of hydrogen peroxide (H(2)O(2)) are produced in wounds and is required for optimal healing. Yet at the same time, there is evidence that excessive oxidative damage is correlated with poor-healing wounds. In this paper, we seek to determine whether topical application of H(2)O(2) can modulate wound healing and if its effects are related to oxidative damage. Using a C57BL/6 mice excision wound model, H(2)O(2) was found to enhance angiogenesis and wound closure at 10 mM but retarded wound closure at 166 mM. The delay in closure was also associated with decreased connective tissue formation, increased MMP-8 and persistent neutrophil infiltration. Wounding was found to increase oxidative lipid damage, as measured by F(2)-isoprostanes, and nitrative protein damage, as measured by 3-nitrotyrosine. However H(2)O(2) treatment did not significantly increase oxidative and nitrative damage even at concentrations that delay wound healing. Hence the detrimental effects of H(2)O(2) may not involve oxidative damage to the target molecules studied

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Effects of hydrogen peroxide on wound healing in mice in relation to oxidative damage.

Loo Alvin Eng Kiat

Wong Yee Ting

Ho Rongjian

Wasser Martin

Du Tiehua

Ng Wee Thong

Halliwell Barry

Public Library of Science (PLOS)

Effects of Hydrogen Peroxide on Wound Healing in Mice in Relation to Oxidative Damage

<div>It has been established that low concentrations of hydrogen peroxide (H2O2) are produced in wounds and is required for optimal healing. Yet at the same time, there is evidence that excessive oxidative damage is correlated with poor-healing wounds. In this paper, we seek to determine whether topical application of H2O2 can modulate wound healing and if its effects are related to oxidative damage. Using a C57BL/6 mice excision wound model, H2O2 was found to enhance angiogenesis and wound closure at 10 mM but retarded wound closure at 166 mM. The delay in closure was also associated with decreased connective tissue formation, increased MMP-8 and persistent neutrophil infiltration. Wounding was found to increase oxidative lipid damage, as measured by F2-isoprostanes, and nitrative protein damage, as measured by 3-nitrotyrosine. However H2O2 treatment did not significantly increase oxidative and nitrative damage even at concentrations that delay wound healing. Hence the detrimental effects of H2O2 may not involve oxidative damage to the target molecules studied. </div

Alvin Eng Kiat Loo (120772)

Yee Ting Wong (120775)

Rongjian Ho (120779)

Martin Wasser (120782)

Tiehua Du (120784)

Wee Thong Ng (120786)

Barry Halliwell (120788)

The Francis Crick Institute

It has been established that low concentrations of hydrogen peroxide (H2O2) are produced in wounds and is required for optimal healing. Yet at the same time, there is evidence that excessive oxidative damage is correlated with poor-healing wounds. In this paper, we seek to determine whether topical application of H2O2 can modulate wound healing and if its effects are related to oxidative damage. Using a C57BL/6 mice excision wound model, H2O2 was found to enhance angiogenesis and wound closure at 10 mM but retarded wound closure at 166 mM. The delay in closure was also associated with decreased connective tissue formation, increased MMP-8 and persistent neutrophil infiltration. Wounding was found to increase oxidative lipid damage, as measured by F2-isoprostanes, and nitrative protein damage, as measured by 3-nitrotyrosine. However H2O2 treatment did not significantly increase oxidative and nitrative damage even at concentrations that delay wound healing. Hence the detrimental effects of H2O2 may not involve oxidative damage to the target molecules studied

Alvin Eng

Kiat Loo

CiteSeerX

Crossref

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Effects of Hydrogen Peroxide on Wound Healing in Mice in Relation to Oxidative Damage

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