10.1371/journal.pone.0087591PLoS ONE91-POLN

A Barbáchano

AK Whitfield

AM Loong

B Sardella

BJ Smith

C Bodinier

C Lorin-Nebel

C Lytle

CA Loretz

CR Taylor

D Kultz

D Kültz

DF Fiol

DH Evans

Eei Yin Lui

EH Larsen

G Laverty

H Takahashi

J Hiroi

JL Horng

JM Whittamore

JM Wilson

Jonathan M. Wilson

Josep V. Planas

K Link

M Grosell

N Ouattara

PP Hwang

Qingsong Lin

RJ Gonzalez

S Hamann

S Namimatsu

S Watanabe

SF Gregório

Siew Hong Lam

SJ Reshkin

Toong Jin Lam

Yuen Kwong Ip

Zhengjun Li

PubMed

<div>The ability of euryhaline Mozambique tilapia to tolerate extreme environmental salinities makes it an excellent model for investigating iono-regulation. This study aimed to characterize and fill important information gap of the expression levels of key ion transporters for Na+ and Cl− in the gill and esophageal-gastrointestinal tract of Mozambique tilapia acclimated to freshwater (0 ppt), seawater (30 ppt) and hypersaline (70 ppt) environments. Among the seven genes studied, it was found that nkcc2, nkcc1a, cftr, nka-α1 and nka-α3, were more responsive to salinity challenge than nkcc1b and ncc within the investigated tissues. The ncc expression was restricted to gills of freshwater-acclimated fish while nkcc2 expression was restricted to intestinal segments irrespective of salinity challenge. Among the tissues investigated, gill and posterior intestine were found to be highly responsive to salinity changes, followed by anterior and middle intestine. Both esophagus and stomach displayed significant up-regulation of nka-α1 and nka-α3, but not nkcc isoforms and cftr, in hypersaline-acclimated fish suggesting a response to hypersalinity challenge and involvement of other forms of transporters in iono-regulation. Changes in gene expression levels were partly corroborated by immunohistochemical localization of transport proteins. Apical expression of Ncc was found in Nka-immunoreactive cells in freshwater-acclimated gills while Nkcc co-localized with Nka-immunoreactive cells expressing Cftr apically in seawater- and hypersaline-acclimated gills. In the intestine, Nkcc-stained apical brush border was found in Nka-immunoreactive cells at greater levels under hypersaline conditions. These findings provided new insights into the responsiveness of these genes and tissues under hypersalinity challenge, specifically the posterior intestine being vital for salt absorption and iono-osmoregulation in the Mozambique tilapia; its ability to survive in hypersalinity may be in part related to its ability to up-regulate key ion transporters in the posterior intestine. The findings pave the way for future iono-regulatory studies on the Mozambique tilapia esophageal-gastrointestinal tract.</div

Zhengjun Li (31438)

Eei Yin Lui (518225)

Jonathan M. Wilson (518226)

Yuen Kwong Ip (518227)

Qingsong Lin (28601)

Toong Jin Lam (518228)

Siew Hong Lam (102343)

The Francis Crick Institute

Expression of Key Ion Transporters in the Gill and Esophageal-Gastrointestinal Tract of Euryhaline Mozambique Tilapia Oreochromis mossambicus Acclimated to Fresh Water, Seawater and Hypersaline Water

Li Zhengjun

Lui Eei Yin

Wilson Jonathan M.

Ip Yuen Kwong

Lin Qingsong

Lam Toong Jin

Lam Siew Hong

Public Library of Science (PLOS)

 Acclimated to Fresh Water, Seawater and Hypersaline Water

Crossref

PLoS ONE

Expression of Key Ion Transporters in the Gill and Esophageal-Gastrointestinal Tract of Euryhaline Mozambique Tilapia Oreochromis mossambicus Acclimated to Fresh Water, Seawater and Hypersaline Water

The ability of euryhaline Mozambique tilapia to tolerate extreme environmental salinities makes it an excellent model for investigating iono-regulation. This study aimed to characterize and fill important information gap of the expression levels of key ion transporters for Na(+) and Cl(-) in the gill and esophageal-gastrointestinal tract of Mozambique tilapia acclimated to freshwater (0 ppt), seawater (30 ppt) and hypersaline (70 ppt) environments. Among the seven genes studied, it was found that nkcc2, nkcc1a, cftr, nka-α1 and nka-α3, were more responsive to salinity challenge than nkcc1b and ncc within the investigated tissues. The ncc expression was restricted to gills of freshwater-acclimated fish while nkcc2 expression was restricted to intestinal segments irrespective of salinity challenge. Among the tissues investigated, gill and posterior intestine were found to be highly responsive to salinity changes, followed by anterior and middle intestine. Both esophagus and stomach displayed significant up-regulation of nka-α1 and nka-α3, but not nkcc isoforms and cftr, in hypersaline-acclimated fish suggesting a response to hypersalinity challenge and involvement of other forms of transporters in iono-regulation. Changes in gene expression levels were partly corroborated by immunohistochemical localization of transport proteins. Apical expression of Ncc was found in Nka-immunoreactive cells in freshwater-acclimated gills while Nkcc co-localized with Nka-immunoreactive cells expressing Cftr apically in seawater- and hypersaline-acclimated gills. In the intestine, Nkcc-stained apical brush border was found in Nka-immunoreactive cells at greater levels under hypersaline conditions. These findings provided new insights into the responsiveness of these genes and tissues under hypersalinity challenge, specifically the posterior intestine being vital for salt absorption and iono-osmoregulation in the Mozambique tilapia; its ability to survive in hypersalinity may be in part related to its ability to up-regulate key ion transporters in the posterior intestine. The findings pave the way for future iono-regulatory studies on the Mozambique tilapia esophageal-gastrointestinal tract

Jonathan M Wilson

Directory of Open Access Journals

Expression of key ion transporters in the gill and esophageal-gastrointestinal tract of euryhaline Mozambique tilapia Oreochromis mossambicus acclimated to fresh water, seawater and hypersaline water.

Adaptation of teleosts to very high salinity. Comp Biochem Physiol A Mol Integr Physiol 163:1–6.

Alvarez-Leefmans FJ (2010) Cotransport of water by the Na+-K+-2Cl(2)c o t r a n s p o r t e rN K C C 1i n mammalian epithelial cells.

Analysis of the sodium recirculation theory of solute-coupled water transport in small intestine.

Antigen retrieval for immunohistochemistry: Status and need for greater standardization.

Basolateral glucose transport by intestine of teleost, Oreochromis mossambicus.

Biochemical characterization of isolated branchial mitochondria-rich cells of Oreochromis mossambicus acclimated to freshwater or hyperhaline seawater.

Both seawater acclimation and environmental ammonia exposure lead to increases in mRNA expression and protein abundance of Na +:K +:2Cl 2 cotransporter in the gills of the climbing perch, Anabas testudineus.

Changes in gill ionocyte morphology and function following transfer from fresh to hypersaline waters in the tilapia Sarotherodon melanotheron.

Chloride transport in mitochondrion-rich cells of euryhaline tilapia (Oreochromis mossambicus) larvae.

Distribution and diversity of Na-K-Cl cotransport proteins: a study with monoclonal antibodies.

Electroneutral cation-Cl-cotransporters NKCC2b and NCCb expressed in the intestinal tract of Japanese eel Anguilla japonica. Comparative Biochemistry and Physiology 159:427–435.

Encarnac ¸a ˜o

Evidences for an apical Na-Cl cotransporter involved in ion uptake in a teleost fish.

Expression of glucocorticoid receptor in the intestine of a euryhaline teleost, the Mozambique tilapia (Oreochromis mossambicus): effect of seawater exposure and cortisol treatment. Life Sci 78:2329–2335.

Extracellular calcium-sensing receptor distribution in osmoregulatory and endocrine tissues of the tilapia.

Extreme environments: hypersaline, alkaline and ion-poor waters.

Gross morphology and topography of the adult intestinal tract of the tilapian fish, Oreochromis niloticus L. Cells Tissues Organs 166:294–303.

Influence of salinity on the localization and expression of the CFTR chloride channel in the ionocytes of Dicentrarchus labrax during ontogeny.

Intestinal anion exchange in marine fish osmoregulation.

Intestinal glucose transport and salinity adaptation in a euryhaline teleost.

Ion regulation in fish gills: recent progress in the cellular and molecular mechanisms.

Ku ¨ltz D

Long-term acclimation of the teleost Oreochromis mossambicus to various salinities: two different strategies in mastering hypertonic stress.

Mitochondria rich (MR) cells and the activities of the Na+/K+-ATPase and carbonic anhydrase in the gill and opercular epithelium of Oreochromis mossambicus adapted to various salinities.

Modulation of branchial ion transport protein expression by salinity in glass eels (Anguilla anguilla L.).

Morphology of Branchial Ionocytes. In:

Ordo ´n ˜ez-Mora ´n P, Garcı ´a JM, Sa ´nchez A,

Osmoregulation and epithelial water transport: lessons from the intestine of marine teleost fish.

Physiological, biochemical, and morphological indicators of osmoregulatory stress in ‘California’ Mozambique tilapia (Oreochromis mossambicus6O. urolepis hornorum) exposed to hypersaline water.

Prolactin receptor and proliferating/apoptotic cells in esophagus of the Mozambique tilapia (Oreochromis mossambicus) in fresh water and in seawater.

Reversing the effects of formalin fixation with citraconic anhydride and heat: A universal antigen retrieval method.

Seawater and freshwater challenges affect the insulin-like growth factors IGF-I and IGF-II in liver and osmoregulatory organs of the tilapia.

The distribution of the freshwater cichlid Sarotherodon mossambicus (Peters) in estuarine systems.

The multifunctional fish gill: dominant site of gas exchange, osmoregulation, acid–base regulation, and excretion of nitrogenous waste. Physiol Rev 85:97–177.

The Na+/K+/ 2Cl2 cotransporter in the sea bass Dicentrarchus labrax during ontogeny: involvement in osmoregulation.

The physiology of hyper-salinity tolerance in teleost fish: a review.

The Role of Feeding in

The role of the gastrointestinal tract in salt and water balance.

Water permeability of Na+-K+-2Cl2 cotransporters in mammalian epithelial cells.

http://scholarbank.nus.edu.sg/bitstream/10635/100643/1/2014-expression_key_ion_transporters_gill-pub.pdf

Expression of key ion transporters in the gill and esophageal- gastrointestinal tract of euryhaline mozambique tilapia oreochromis mossambicus acclimated to fresh water, seawater and hypersaline water

Expression of key ion transporters in the gill and esophageal- gastrointestinal tract of euryhaline mozambique tilapia oreochromis mossambicus acclimated to fresh water, seawater and hypersaline water

Abstract

Similar works

Full text

Available Versions

The Francis Crick Institute

Public Library of Science (PLOS)

Public Library of Science (PLOS)

Crossref

Directory of Open Access Journals