Epithelial barrier dysfunction is a significant factor in many allergic diseases, including eosinophilic esophagitis (EoE).
Infiltrating leukocytes and tissue adaptations increase metabolic demands and decrease oxygen availability at barrier
surfaces. Understanding of how these processes impact barrier is limited, particularly in allergy. Here, we identified a
regulatory axis whereby the oxygen-sensing transcription factor HIF-1α orchestrated epithelial barrier integrity, selectively
controlling tight junction CLDN1 (claudin-1). Prolonged experimental hypoxia or HIF1A knockdown suppressed HIF-1α–
dependent claudin-1 expression and epithelial barrier function, as documented in 3D organotypic epithelial cultures. L2-IL5OXA
mice with EoE-relevant allergic inflammation displayed localized eosinophil oxygen metabolism, tissue hypoxia, and impaired
claudin-1 barrier via repression of HIF-1α/claudin-1 signaling, which was restored by transgenic expression of esophageal
epithelial-targeted stabilized HIF-1α. EoE patient biopsy analysis identified a repressed HIF-1α/claudin-1 axis, which was
restored via pharmacologic HIF-1α stabilization ex vivo. Collectively, these studies reveal HIF-1α’s critical role in maintaining
barrier and highlight the HIF-1α/claudin-1 axis as a potential therapeutic target for EoE