A Ahmad

A Brunello

AA Laitiff

AC Akinmoladun

AM Dieye

AM Moustafa

B Schmidt

B Valderrama

Bradley G. Stiles

C Circosta

C Steiner

CD Teixeira de Freitas

CM Maier

CM Vachon

D Malejka-Gignati

DP Souza

F Li

Feng Li

G Prasad

G Sethi

Gautam Sethi

GL Gierach

GM Rishton

H Greenlee

HI Magalhaes

IA Muraina

J Vecanova

JD Flom

JM De Lima

JS Mossa

JS Oliveira

JV Kamath

JV Lima-Filho

JW Forstrom

K Heusinger

K Tikoo

LE Kelemen

LH Johansson

M Pandey

M Shahi

MA Louis Jeune

MA Sovak

Maria G. Castro

MK Shanmugam

MM Bradford

MW Roomi

N Andrieu

N Tour

Narayana Moorthy Anandi

NB Kumar

NO Ali

P Terrier

PC Cogswell

Peramaiyan Rajendran

PK Jain

R Perumal Samy

R Verma

Ramar Perumal Samy

RC Bargou

RP Samy

RWE Clarkson

S Badami

S Guo

S Gupta

SA Narod

Savarimuthu Ignacimuthu

SK Sagiv

ST Omaye

SW Lowe

SZ Hasan

T Khalili-Azad

TA Buchholz

TA Morsy

Vincent T. K. Chow

VL Kumar

WH Habig

Y Lin

Y Murti

YJ Xiang

PLoS ONE

PubMed

Breast cancer is the most common cancer among women. To date, improvements in hormonal and cytotoxic therapies have not yet led to a sustained remission or cure. In the present study, we investigated the in vitro and in vivo antitumor activities of a novel Calotropis procera protein (CP-P) isolated from root bark. CP-P protein inhibited the proliferation and induced apoptosis of breast cancer cells through the suppression of nuclear factor kappaB (NF-kB) activation. CP-P, when administered individually or in combination with cyclophosphamide (CYC, 0.2 mg/kg) to rats with 7, 12-dimethyl benz(a)anthracene (DMBA)-induced breast cancer decreased tumor volume significantly without affecting the body weight. To elucidate the anticancer mechanism of CP-P, antioxidant activities such as superoxide dismutase (SOD), catalase (CAT), glutathione-s-transferase (GST) and non-enzymatic antioxidant - reduced glutathione (GSH), vitamin E and C generation in the breast were analyzed by various assays. SOD, CAT, GST, GSH, vitamin E and C levels were high in combination-treated groups (CP-P+CYC) versus the CYC alone-treated groups. Also, the combination was more effective in down-regulating the expression of NF-kB-regulated gene products (cyclin D1 and Bcl-2) in breast tumor tissues. Our findings indicate that CP-P possesses significant antitumor activity comparable to a commonly used anticancer drug, cyclophosphamide, and may form the basis of a novel therapy for breast cancer

Bradley G Stiles

Vincent T K Chow

Directory of Open Access Journals

Identification of a novel Calotropis procera protein that can suppress tumor growth in breast cancer through the suppression of NF-κB pathway.

Crossref

Identification of a Novel Calotropis procera Protein That Can Suppress Tumor Growth in Breast Cancer through the Suppression of NF-κB Pathway

<div>Breast cancer is the most common cancer among women. To date, improvements in hormonal and cytotoxic therapies have not yet led to a sustained remission or cure. In the present study, we investigated the in vitro and in vivo antitumor activities of a novel Calotropis procera protein (CP-P) isolated from root bark. CP-P protein inhibited the proliferation and induced apoptosis of breast cancer cells through the suppression of nuclear factor kappaB (NF-kB) activation. CP-P, when administered individually or in combination with cyclophosphamide (CYC, 0.2 mg/kg) to rats with 7, 12-dimethyl benz(a)anthracene (DMBA)-induced breast cancer decreased tumor volume significantly without affecting the body weight. To elucidate the anticancer mechanism of CP-P, antioxidant activities such as superoxide dismutase (SOD), catalase (CAT), glutathione-s-transferase (GST) and non-enzymatic antioxidant - reduced glutathione (GSH), vitamin E and C generation in the breast were analyzed by various assays. SOD, CAT, GST, GSH, vitamin E and C levels were high in combination-treated groups (CP-P+CYC) versus the CYC alone-treated groups. Also, the combination was more effective in down-regulating the expression of NF-kB-regulated gene products (cyclin D1 and Bcl-2) in breast tumor tissues. Our findings indicate that CP-P possesses significant antitumor activity comparable to a commonly used anticancer drug, cyclophosphamide, and may form the basis of a novel therapy for breast cancer. </div

Ramar Perumal Samy (109717)

Peramaiyan Rajendran (109720)

Feng Li (30515)

Narayana Moorthy Anandi (109724)

Bradley G. Stiles (109727)

Savarimuthu Ignacimuthu (109729)

Gautam Sethi (109732)

Vincent T. K. Chow (109735)

The Francis Crick Institute

Identification of a Novel Calotropis procera Protein That Can Suppress Tumor Growth in Breast Cancer through the Suppression of NF-κB Pathway

Samy Ramar Perumal

Rajendran Peramaiyan

Li Feng

Anandi Narayana Moorthy

Stiles Bradley G.

Ignacimuthu Savarimuthu

Sethi Gautam

Chow Vincent T. K.

Public Library of Science (PLOS)

 Protein That Can Suppress Tumor Growth in Breast Cancer through the Suppression of NF-κB Pathway

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http://dx.doi.org/10.1371/journal.pone.0048514

Identification of a Novel Calotropis procera Protein That Can Suppress Tumor Growth in Breast Cancer through the Suppression of NF-κB Pathway

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