Lying cues observed by men and women were investigated by a combination of a 2x2 mixed subjects design and a correlational design. Fifty-nine male and 68 female fluent English-speaking college students older than 18 years of age were tasked with completing a 64-item questionnaire and observing two video clips. The participants completed the questionnaire for a self-assessment of the perception of their own lying cues, observed the video clips, and then completed the questionnaire for an assessment of the lying cues observed in the videos. Independent sample t-test results indicated that, for self-assessment of lying cues, there was a statistically significant difference in the speech behavior and facial behavior lying cues of men and women. Pearson correlation indicated that there was a correlation between the lying cues and gender. Results are discussed in terms of self-other theory and gender differences in nonverbal behavior