Arunkumar S

Davis RH

Debiasi C

Deng XH

Lila MA

Lindsey K

M. Guleria

McKeown E.

P. Chaudhary

Sharma V

Sims P

Strickland FM

Tanabe MJ.

V. Sharma

Vanisree M

Wang LC.

Xiaohong L

Yeoman MM

Zenk MH.

Aloe vera(Liliaceae) is well known for its medicinal properties. It contains flavonoids, saponins, tannins, alkaloids and other compounds which exhibit medicinal, cosmetic and pharmacological properties. There is a lack of production of aloe leaf to meet the industry demand and therefore the means to facilitate large-scale aloe cultivation schemes need to be developed. Because sexual reproduction by seeds is almost ineffective inAloe veradue to male sterility and vegetative propagation by offshoots is only possible during the growing seasons, there is a need to develop a propagation method to facilitate large-scale cultivation.In vitroregeneration of medicinal plants is important and there is the potential for the production of high-quality plant-based medicine. In the present study, an attempt has been made to enhancein vitrocaulogenesis and aloin content in plants in the presence of a precursor (tryptophan) in the nutrient media. Forin vitroculture establishment and shoot bud multiplication, MS basal media were used supplemented with different concentrations and different combinations of growth regulators such as BAP (6-benzylaminopurine) and IAA (3-indole acetic acid). Shoot proliferation was optimal in MS medium containing 2.0 mg l−1BAP. For evaluation ofin vitroenhancement of aloin production the precursor tryptophan was added to the nutrient media at different levels (5, 10, 15 and 20 mg l−1). Addition of 20 mg l−1tryptophan induced a 2.43-fold increase in aloin content in multiple shoots cultures ofAloe barbadensis.</jats:p

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Israel Journal of Plant Sciences

Enhanced in vitro caulogenesis and quantitative estimation of aloin through HPLC in Aloe vera

Enhanced in vitro caulogenesis and quantitative estimation of aloin through HPLC in Aloe vera

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