Hand-hygiene is a very critical issue for both food handling and processing industry and health care service providers. Poor hand-hygiene practice can easily lead to foodborne illness or large scale decease transmission. In this research, an automatic tracking and monitoring system was developed that used a 3D camera for hand washing and hands motion detection and a sensor-based monitoring system for hand-hygiene activities evaluation. An active Wi-Fi portable Radio Frequency Identification (RFID) tag was used for personal ID tracking. The effective hand washing time, soaping time were measured based on the hands motion detection and hand movement tracking. Water temperature, water flow, paper towel, soap and hand sanitizer usage were also measured for each hand washing event. All the data were forwarded to a system server for data recording, storage and management. Preliminary test data were collected to evaluate the system performance. The results showed that the system could effectively collect most of the hand-hygiene related factors including hand-hygiene product usage, hand washing time and soap lathering time for hand-hygiene evaluation.Biosystems & Agricultural Engineerin