The rhizome of Shwe-pan was collected from Pyipan village, Kyaukse Township, Mandalay Region. The preliminary detection of phytochemical compounds presents in rhizome extracted was carried out by phytochemical tests. The elemental analysis of rhizome extracted was also investigated by Energy Dispersive X-ray Fluorescence (EDXRF) Spectroscopy. An experiment has been synthesized the copper oxide nanoparticles using an aqueous solution extracted from the rhizome of Shwe-pan. The size distribution of nanoparticles surface and morphology of copper oxide nanoparticles were determined by X-ray diffraction (XRD) method and Scanning Electron Microscopy (SEM) method. The characteristic absorption peaks of copper oxide nanoparticles were analysis by Fourier Transform Infrared (FTIR) Spectroscopy. Antimicrobial activities of synthesized copper oxide nanoparticles were examined by agar well diffusion method on tested microorganisms. The synthesis of nanoparticles using the rhizome extract is an affordable method and it reduces the environmental pollution