Insulin Receptor Substrate-1 Gene (G972R) Polymorphism and Insulin Resistance in Overweight and Obese Type 2 Diabetes Mellitus Patients

Abstract

The insulin receptor substrate-1 gene (IRS-1) gene has been considered a candidate for insulin resistance in type-2 diabetes and coronary artery disease. The most common IRS-1 variant, a glycine to arginine change at codon 972(G972R) is more prevalent among subjects who have features of insulin resistance syndrome associated with type 2 diabetes patients. The aim of the present study was to determine the insulin receptor substrate-1 gene (G972R) polymorphism and insulin resistance in overweight and obese type 2 diabetes mellitus patients. The genomic DNA of the subjects was amplified by polymerase chain reaction (PCR) and digested by restriction fragment length polymorphism (RFLP) with BstN1 used for codon 972.</p