A Hallare

A Knoll-Gellida

A Mode

AB Rogers

AK Roy

AT Nguyen

B Desvergne

B Gao

B Ruggeri

BD Robison

C Handschin

C Wang

C Wen

C Wolfrum

C Zhao

CA Wiwi

CH Pham

CM Small

CM Troxel

CY Ung

DJ Waxman

DL Howarth

EM Santos

EM Sternberg

FW Goetz

H Dell

H Gao

H Wang

Hongyan Xu

Huaien Luo

IV Deaciuc

J Correale

J van Riggelen

JL Hoffmann

JL Wang-Buhler

KJ Groh

L Levi

L Yafang

LK Davis

LN Moens

M Gao

M Stoffel

MD Robinson

ME Baker

P Kling

R Sreenivasan

R. Krishna Murthy Karuturi

S Curado

S Hashimoto

S Zeng

S Zhao

SE Hook

SH Lam

Siew Hong Lam

TF Holth

U Kausch

W Cheng

W Huang da

W Zhang

Weiling Zheng

X Liu

X Taboada

X Tan

X Yang

Y Li

Y Shang

Y Tong

Y Weng

Z Li

Zhanjiang Liu

Zhiyuan Gong

ZJ Li

PLoS ONE

PubMed

<div><p>The liver is one of the most sex-dimorphic organs in both oviparous and viviparous animals. In order to understand the molecular basis of the difference between male and female livers, high-throughput RNA-SAGE (serial analysis of gene expression) sequencing was performed for zebrafish livers of both sexes and their transcriptomes were compared. Both sexes had abundantly expressed genes involved in translation, coagulation and lipid metabolism, consistent with the general function of the liver. For sex-biased transcripts, from in addition to the high enrichment of vitellogenin transcripts in spawning female livers, which constituted nearly 80% of total mRNA, it is apparent that the female-biased genes were mostly involved in ribosome/translation, estrogen pathway, lipid transport, etc, while the male-biased genes were enriched for oxidation reduction, carbohydrate metabolism, coagulation, protein transport and localization, etc. Sexual dimorphism on xenobiotic metabolism and anti-oxidation was also noted and it is likely that retinol x receptor (RXR) and liver x receptor (LXR) play central roles in regulating the sexual differences of lipid and cholesterol metabolisms. Consistent with high ribosomal/translational activities in the female liver, female-biased genes were significantly regulated by two important transcription factors, Myc and Mycn. In contrast, Male livers showed activation of transcription factors Ppargc1b, Hnf4a, and Stat4, which regulate lipid and glucose metabolisms and various cellular activities. The transcriptomic responses to sex hormones, 17β-estradiol (E2) or 11-keto testosterone (KT11), were also investigated in both male and female livers and we found that female livers were relatively insensitive to sex hormone disturbance, while the male livers were readily affected. E2 feminized male liver by up-regulating female-biased transcripts and down-regulating male-biased transcripts. The information obtained in this study provides comprehensive insights into the sexual dimorphism of zebrafish liver transcriptome and will facilitate further development of the zebrafish as a human liver disease model.</p> </div

Weiling Zheng (102342)

Hongyan Xu (29047)

Siew Hong Lam (102343)

Huaien Luo (102346)

R. Krishna Murthy Karuturi (102350)

Zhiyuan Gong (51844)

The Francis Crick Institute

Transcriptomic Analyses of Sexual Dimorphism of the Zebrafish Liver and the Effect of Sex Hormones

Crossref

Zheng Weiling

Xu Hongyan

Lam Siew Hong

Luo Huaien

Karuturi R. Krishna Murthy

Gong Zhiyuan

Public Library of Science (PLOS)

The liver is one of the most sex-dimorphic organs in both oviparous and viviparous animals. In order to understand the molecular basis of the difference between male and female livers, high-throughput RNA-SAGE (serial analysis of gene expression) sequencing was performed for zebrafish livers of both sexes and their transcriptomes were compared. Both sexes had abundantly expressed genes involved in translation, coagulation and lipid metabolism, consistent with the general function of the liver. For sex-biased transcripts, from in addition to the high enrichment of vitellogenin transcripts in spawning female livers, which constituted nearly 80% of total mRNA, it is apparent that the female-biased genes were mostly involved in ribosome/translation, estrogen pathway, lipid transport, etc, while the male-biased genes were enriched for oxidation reduction, carbohydrate metabolism, coagulation, protein transport and localization, etc. Sexual dimorphism on xenobiotic metabolism and anti-oxidation was also noted and it is likely that retinol x receptor (RXR) and liver x receptor (LXR) play central roles in regulating the sexual differences of lipid and cholesterol metabolisms. Consistent with high ribosomal/translational activities in the female liver, female-biased genes were significantly regulated by two important transcription factors, Myc and Mycn. In contrast, Male livers showed activation of transcription factors Ppargc1b, Hnf4a, and Stat4, which regulate lipid and glucose metabolisms and various cellular activities. The transcriptomic responses to sex hormones, 17β-estradiol (E2) or 11-keto testosterone (KT11), were also investigated in both male and female livers and we found that female livers were relatively insensitive to sex hormone disturbance, while the male livers were readily affected. E2 feminized male liver by up-regulating female-biased transcripts and down-regulating male-biased transcripts. The information obtained in this study provides comprehensive insights into the sexual dimorphism of zebrafish liver transcriptome and will facilitate further development of the zebrafish as a human liver disease model

R Krishna Murthy Karuturi

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https://figshare.com/articles/Transcriptomic_Analyses_of_Sexual_Dimorphism_of_the_Zebrafish_Liver_and_the_Effect_of_Sex_Hormones__/114420

Transcriptomic Analyses of Sexual Dimorphism of the Zebrafish Liver and the Effect of Sex Hormones

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