Not AvailableA comprehensive experiment was conducted to study the accumulation pattern and determination of three important bioactive compounds namely withaferin-A (WA), 12-deoxywithastramonolide (WO) and withanolide-A (WD) and its determination by the liquid chromatography/electrospray ionization tandem mass spectrometry (LC–ESI-MS-MS) method in root, stem, fruits and leaves of Withania somnifera. A rapid and sensitive LC–ESI-MS-MS method was developed and validated for the determination of these three important bioactive compounds, having same molecular weight. The multiplereactionmonitoringmethodwasestablishedbytwotransitionsforeachanalyteandintense transitionusedfor quantification. Separation ofthethree analyteswas achievedwithin aruntimeof 5 min on an RP-18 column using a mobile phase consisting of acetonitrile and 0.1% acetic acid in water in an isocratic condition. The developed method was validated as per the ICH guidelines. The developed method was found to be suitable for identification and quantification of WA, WO andWD indifferent plantparts suchas roots, stems, fruitsand leaves ofW.somnifera. Theaccumulation of WAwas highest in leaves samples (8.84±0.37 mg/g) and it was 2.23, 5.85 and 27.26 times higherthanitsconcentrationinfruits,stemsandroots,respectively.WOandWDcontentswerehighest (0.44±0.016 and 0.72±0.016 mg/g, respectively) in root.Not Availabl
