Not AvailableMucuna pruriens(L.) is an important underutilized legume medicinal plant. It is an herbaceous annual twiningclimber. Its seeds are used to extract the L-Dopa (L-3, 4-dihydroxyphenylalanine) to the preparation of the drugsand are used in the treatment of Parkinson’s disease. Mucuna seeds are rich source of L-Dopa in addition toprotein and other minerals. In the present study estimation of L-Dopa and other biochemical traits in 58 gen-otypes of velvet bean has been analyzed to identify the genotypes having maximum seed yield coupled with highL-Dopa to the preparation of drugs. The L-Dopa is varied significantly from 2.94 to 6.91% among mucunagenotypes. The levels of total phenol content and tannins varied significantly among genotypes and ranged from27.73 to 103.50 mg/100 g and 0.18 to 0.96 mg/100 g DW, respectively. The protein content in the estimatedgenotypes of Mucuna seed greatly varied from 19.08% to 38.18% with a mean value of 28.16%. Significantlymaximum L-Dopa, total phenol content, total tannin, crude protein, fat and carbohydrate was recorded in IIHRMP 62-1 (6.91%), IIHR MP 62-2 (103.50 mg/100 g), IIHR MP 63-1 (0.96 mg/100 g), IIHR MP-74 (38.18%), IIHRMP 95 (7.54%) and IIHR MP 62-3 (64.36%), respectively. Significantly low L-Dopa, total phenol content, totaltannin found in IC 332432 (2.94%), IIHR MP 89-1 (27.73 mg/100 g), IIHR MP 10 (0.183 mg/100 g), respec-tively. Biochemical traits viz., crude protein, fat and carbohydrate found less in EC 17827 (19.08%), IIHR MP 62-3 (3.10%) and IIHR MP 74 (47.81%), respectively. Cluster analysis based on biochemical data revealed that thecluster I showed highest mean value for L-Dopa and total phenol content, cluster III for total tannin and clusterVI for crude protein. The results showed that intra cluster distance was maximum in cluster VI (167.51) andmaximum inter cluster distance was between cluster I and V (2014.94). Total phenol content was the maincontributor to the total genetic divergence. The traits fat and L-Dopa were moderately contributing and car-bohydrate was least contributing to the total divergence of the estimated biochemical traits. The genotypesbelonging to the cluster with maximum inter cluster distance are genetically more divergent and these genotypescould be used in the hybridization programme of velvet bean to evolve high L-Dopa type to meet the demand of drug industry.Not Availabl