Damrongwatanapokin, S.

Du, Q.

Fenner, B.J.

He, Q.

Kwang, J.

Limlamthong, Y.

Manopo, I.

Nwe, N.

Spencer, L.

PubMed

Nitar Nwe

Qigai He

Sudarat Damrongwatanapokin

Qingyun Du

Ivanus Manopo

Yukol Limlamthong

Beau Fenner

Lynn Spencer

Jimmy Kwang

Springer - Publisher Connector

Expression of hemagglutinin protein from the avian influenza virus H5N1 in a baculovirus/insect cell system significantly enhanced by suspension culture

Abstract Background Prevention of a possible avian influenza pandemic necessitates the development of rapid diagnostic tests and the eventual production of a vaccine. Results For vaccine production, hemagglutinin (HA1) from avian influenza H5N1 was expressed from a recombinant baculovirus. Recombinant HA1 was expressed in monolayer or suspension culture insect cells by infection with the recombinant baculovirus. The yield of rHA1 from the suspension culture was 68 mg/l, compared to 6 mg/l from the monolayer culture. Immunization of guinea pigs with 50 μg of rHA1 yielded hemagglutinin inhibition and virus neutralization titers of 1:160 after two times vaccination with rHA1 protein. Conclusion Thus, the production of rHA1 using an insect suspension cell system provides a promising basis for economical production of a H5 antigen.</p

Spencer Lynn

Fenner Beau

Limlamthong Yukol

Manopo Ivanus

Du Qingyun

Damrongwatanapokin Sudarat

He Qigai

Nwe Nitar

Kwang Jimmy

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http://scholarbank.nus.edu.sg/bitstream/10635/24714/1/2006-Expression_hemagglutinin_protein_avian_influenza-published.pdf

Expression of hemagglutinin protein from the avian influenza virus H5N1 in a baculovirus/insect cell system significantly enhanced by suspension culture

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