Tran, Vy P

English

Georgia State University

REGULATION OF BROWN ADIPOCYTE GENE EXPRESSION BY THE HISTONE 
H3 LYSINE 4 DEMETHYLASE KDM1b 
Vy Tran, Bingzhong Xue, Ph.D, and Hang Shi, Ph.D 
 
ABSTRACT 
 
Brown adipocytes function to dissipate energy as heat through adaptive 
thermogenesis due to the unique expression of uncoupling protein 1 (Ucp1). 
Understanding the molecular mechanisms underlying the brown fat thermogenic program 
may provide insights for the development of therapeutic approaches in the treatment of 
obesity. Most studies investigating the mechanisms underlying brown fat development 
focus on genetic mechanisms; little is known about the epigenetic mechanisms in this 
process. Lysine (K)-specific demethylase 1B (KDM1b) is a histone demethylase that 
specifically demethylates mono- and di-methylated histone H3 lysine 4 (H3K4me1/2), 
which plays an important role in maternal genomic imprinting during oocyte 
development. We found that deleting KDM1b significantly suppressed basal and the 
beta-adrenergic agonist isoproterenol-stimulated Ucp1 in brown adipocyte cell line BAT-
1 cells. Thus, the H3K4me1/2 demethylase KDM1b may be involved in the regulation of 
brown adipocyte thermogenic function.  
 


Regulation of Brown Adipocyte Gene Expression by the Histone 3 Lysine 4 Demethylase KDM1B

ScholarWorks @ Georgia State University

REGULATION OF BROWN ADIPOCYTE GENE EXPRESSION BY THE HISTONE H3 LYSINE 4 DEMETHYLASE KDM1b Vy Tran, Bingzhong Xue, Ph.D, and Hang Shi, Ph.D  ABSTRACT  Brown adipocytes function to dissipate energy as heat through adaptive thermogenesis due to the unique expression of uncoupling protein 1 (Ucp1). Understanding the molecular mechanisms underlying the brown fat thermogenic program may provide insights for the development of therapeutic approaches in the treatment of obesity. Most studies investigating the mechanisms underlying brown fat development focus on genetic mechanisms; little is known about the epigenetic mechanisms in this process. Lysine (K)-specific demethylase 1B (KDM1b) is a histone demethylase that specifically demethylates mono- and di-methylated histone H3 lysine 4 (H3K4me1/2), which plays an important role in maternal genomic imprinting during oocyte development. We found that deleting KDM1b significantly suppressed basal and the beta-adrenergic agonist isoproterenol-stimulated Ucp1 in brown adipocyte cell line BAT-1 cells. Thus, the H3K4me1/2 demethylase KDM1b may be involved in the regulation of brown adipocyte thermogenic function.   

https://scholarworks.gsu.edu/cgi/viewcontent.cgi?article=1642&amp;context=gsurc

Regulation of Brown Adipocyte Gene Expression by the Histone 3 Lysine 4 Demethylase KDM1B

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