Bowman, Holly M

Purvis, Grace

English

Georgia State University

Protein Products from Plants and Pichia: Novel Manufacturing of Analgesics and 
Cannabinoids 
 
Cannabinoids and opiates are widely used classes of pharmaceuticals; unfortunately, these 
drugs have strong psychoactive effects or can be addictive. Our project consists of two ideas, both 
revolving around utilizing bioengineered microorganisms to create non-psychoactive 
cannabinoids and non-addictive analgesics. To achieve this, we developed two projects: (1) 
Manufacturing a protein expression system to produce CBDA synthase in tobacco plants using 
Agrobacterium, (2) Engineering the pGAPα vector system to express the mambalgin in Pichia 
Pastoris as a continuation of the 2014 GSU iGEM project. Simultaneously, we developed a proof 
of concept using horseradish peroxidase. By the end of this project, we hope to have produced a 
synthetic biological system to manufacture pharmaceutical alternatives for patients that suffer from 
diseases such as epilepsy, cancer, or severe chronic pain.  
	


Protein Products from Plants and Pichia: Novel Manufacturing of Analgesics and Cannabinoids

ScholarWorks @ Georgia State University

Protein Products from Plants and Pichia: Novel Manufacturing of Analgesics and Cannabinoids  Cannabinoids and opiates are widely used classes of pharmaceuticals; unfortunately, these drugs have strong psychoactive effects or can be addictive. Our project consists of two ideas, both revolving around utilizing bioengineered microorganisms to create non-psychoactive cannabinoids and non-addictive analgesics. To achieve this, we developed two projects: (1) Manufacturing a protein expression system to produce CBDA synthase in tobacco plants using Agrobacterium, (2) Engineering the pGAPα vector system to express the mambalgin in Pichia Pastoris as a continuation of the 2014 GSU iGEM project. Simultaneously, we developed a proof of concept using horseradish peroxidase. By the end of this project, we hope to have produced a synthetic biological system to manufacture pharmaceutical alternatives for patients that suffer from diseases such as epilepsy, cancer, or severe chronic pain.  	

https://scholarworks.gsu.edu/cgi/viewcontent.cgi?article=1567&amp;context=gsurc

Protein Products from Plants and Pichia: Novel Manufacturing of Analgesics and Cannabinoids

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Georgia State University

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